Description

n-Hydroxysuccinimide Caproic Acid Ester is a key activated ester derivative used as a versatile bioconjugation reagent. Its primary value lies in facilitating efficient and selective amide bond formation under mild aqueous conditions, which is critical for modifying biomolecules. This compound is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require reliable cross-linking agents for protein labeling, peptide synthesis, and surface functionalization.

Application

• Protein and Antibody Conjugation: Used to covalently attach labels (e.g., fluorophores, enzymes), drugs, or other functional groups to lysine residues.
• Biomolecule Immobilization: Enables the stable coupling of proteins, peptides, or oligonucleotides to solid surfaces for biosensor and microarray development.
• Peptide Synthesis: Serves as an activated intermediate for the formation of peptide bonds in solution-phase or solid-phase synthesis strategies.
• Polymer Functionalization: Modifies polymers and hydrogels with bioactive ligands to create advanced biomaterials for tissue engineering and drug delivery.
• Diagnostic Reagent Production: A critical component in the manufacture of enzyme-linked immunosorbent assay (ELISA) kits and other immunoassay reagents.
• Cross-linking Agent: Facilitates the creation of defined protein-protein or protein-small molecule conjugates for structural and functional studies.

Basic Information

Product Name	n-Hydroxysuccinimide Caproic Acid Ester
CAS No.	22102-92-7
Molecular Formula	C10H15NO5
Molecular Weight	229.23 g/mol
Synonyms	NHS-Caproate; 6-[(2,5-Dioxopyrrolidin-1-yl)oxy]-6-oxohexanoic acid; N-Hydroxysuccinimidyl Caproate; Succinimidyl 6-(carboxy)hexanoate; Caproic Acid N-Hydroxysuccinimide Ester; Hexanoic Acid, 6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxo-; NHS-Hexanoate; N-Succinimidyl 6-Carboxyhexanoate
EINECS	Contact for details

Quality Control

Our n-Hydroxysuccinimide Caproic Acid Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccant or under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.