Description

n-Succinimidoxycarbonyl-β-Alanine n-Succinimidyl Ester CAS NO 21994-89-8 is a highly reactive, heterobifunctional crosslinking agent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester group and a carboxyl group. This compound matters for its ability to facilitate the stable conjugation of biomolecules, such as proteins and peptides, to various surfaces, particles, or other molecules under mild aqueous conditions. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, diagnostics development, and pharmaceutical research for creating advanced reagents, assay components, and drug delivery systems.

Application

• Bioconjugation Reagent: For covalently linking amines (e.g., on antibodies, proteins) to carboxylated surfaces, polymers, or nanoparticles.
• Diagnostic Assay Development: Immobilization of capture antibodies or antigens onto carboxyl-functionalized microplates or magnetic beads for ELISA and immunoassays.
• Affinity Chromatography Ligand Coupling: Attachment of proteins or peptides to carboxylated resin supports for purification columns.
• Drug Delivery System Fabrication: Conjugation of targeting ligands (e.g., peptides, antibodies) to the surface of polymeric nanoparticles or liposomes.
• Biomaterial Surface Modification: Functionalizing medical devices or implant surfaces with bioactive molecules to enhance biocompatibility or functionality.
• Fluorescent Probe & Dye Labeling: Synthesis of amine-reactive fluorescent dyes or other probes from carboxylated precursor molecules.

Basic Information

Product Name	n-Succinimidoxycarbonyl-β-Alanine n-Succinimidyl Ester
CAS No.	21994-89-8
Molecular Formula	C11H12N2O7
Molecular Weight	284.22 g/mol
Synonyms	3-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-3-oxopropanoic Acid 2,5-Dioxo-1-pyrrolidinyl Ester; β-Alanine, N-succinimidoxycarbonyl-, N-succinimidyl ester; NHS-β-Alanine-NHS; Succinimidyl 3-[(2,5-dioxo-1-pyrrolidinyl)oxy]-3-oxopropanoate; BIS(N-Succinimidyl) β-Alaninate; β-Alanine bis(N-succinimidyl) ester; BSNHS; Bis(succinimidyl) β-alanate
EINECS	Contact for details

Quality Control

Our n-Succinimidoxycarbonyl-β-Alanine n-Succinimidyl Ester is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.