Description

Bz-D-Arg-Pna Hcl CAS NO 21653-41-8 is a protected peptide derivative, specifically the hydrochloride salt of Nα-Benzoyl-D-arginine p-nitroanilide. This compound serves as a critical chromogenic substrate for the specific detection and kinetic study of serine proteases, most notably trypsin and trypsin-like enzymes. It is an essential reagent for researchers and manufacturers in pharmaceutical development, diagnostic kit production, and biochemical research, where precise enzyme activity measurement is paramount.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of trypsin, thrombin, plasmin, and other serine protease activities.
• Diagnostic Reagent Manufacturing: Key component in the formulation of clinical diagnostic kits for pancreatic function and coagulation disorders.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and protease characterization in academic and industrial R&D laboratories.
• Pharmaceutical Quality Control: Employed in purity and potency testing of enzyme-based pharmaceutical products.
• Proteomics: A tool for studying protease specificity and function within complex biological systems.

Basic Information

Product Name	Bz-D-Arg-Pna Hcl
CAS No.	21653-41-8
Molecular Formula	C20H25N7O4 • HCl
Molecular Weight	487.92 g/mol
Synonyms	Nα-Benzoyl-D-arginine 4-nitroanilide hydrochloride; Bz-D-Arg-pNA • HCl; D-BAPA; Benzoyl-D-arginine p-nitroanilide hydrochloride; BAPNA (D-isomer); N-Benzoyl-D-arginine-4-nitroanilide hydrochloride; Bz-D-Arg-4-NA • HCl; Trypsin substrate (chromogenic, D-form)
EINECS	Contact for details

Quality Control

Our Bz-D-Arg-Pna Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and MS, and specific optical rotation verification to ensure consistency and performance as a high-grade biochemical reagent. Certificates of Analysis (COA) with detailed chromatograms and spectroscopic data are provided to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	-10.0° to -14.0° (c=1 in DMF)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, yellow solution in DMSO or DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.