Description

25-Hydroxyvitamin D2 is a crucial intermediate metabolite of ergocalciferol (Vitamin D2), serving as a primary circulating biomarker for assessing Vitamin D status. This compound is of significant importance in pharmaceutical research and clinical diagnostics for evaluating bone health, calcium homeostasis, and immune function. It is essential for manufacturers of in-vitro diagnostic (IVD) kits, reference standards, and research institutions focused on endocrinology and metabolic diseases.

Application

• Clinical Diagnostics: Critical component in the manufacturing of immunoassay and LC-MS/MS kits for the quantitative measurement of Vitamin D status in human serum/plasma.
• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for method development, validation, and quality control in drug analysis and pharmacokinetic studies.
• Biochemical Research: Vital reagent for studying the Vitamin D metabolic pathway, receptor binding, and its role in cellular processes related to bone metabolism and immune regulation.
• Nutraceutical Analysis: Employed as an analytical standard for the assay and quality control of Vitamin D2-fortified foods and dietary supplements.
• Veterinary Medicine: Used in diagnostic assays to monitor Vitamin D levels and metabolic bone disorders in animals.

Basic Information

Item	Detail
Product Name	25-Hydroxyvitamin D2
CAS No.	21343-40-8
Molecular Formula	C28H44O2
Molecular Weight	412.65 g/mol
Synonyms	25-Hydroxyergocalciferol; 25(OH)D2; Calcifediol D2; 25-Hydroxy Vitamin D2; (3β,5Z,7E,22E)-9,10-Secocholesta-5,7,10(19),22-tetraene-3,25-diol; 25-Hydroxycholecalciferol D2; Previtamin D2 Metabolite; EINECS 244-319-5
EINECS	244-319-5

Quality Control

Our 25-Hydroxyvitamin D2 is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR, and residual solvent analysis to ensure it meets high-purity standards suitable for diagnostic and research use. A Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. For long-term storage, keep desiccated at -20°C or below. Allow the container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Loss on Drying	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.