Description

4-Nitrophenylphosphorylcholine is a specialized biochemical reagent featuring a chromogenic nitrophenyl group linked to a phosphorylcholine moiety. This compound is valued for its role as a substrate in enzymatic assays, particularly for detecting phospholipase activity. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic kit production sectors.

Application

• Enzymatic Substrate: Key substrate for the assay of phospholipase C (PLC) and phospholipase D (PLD) enzymes, where hydrolysis releases the chromogenic 4-nitrophenol.
• Biochemical Research: Used in fundamental research to study phospholipid metabolism, cell signaling pathways, and enzyme kinetics.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential inhibitors or activators of phospholipase enzymes for drug discovery.
• Diagnostic Development: Integral component in the development of colorimetric diagnostic kits for measuring phospholipase activity in clinical or research samples.
• Quality Control: Serves as a reference standard in the quality control of enzyme preparations and diagnostic reagents within biotechnology.

Basic Information

Product Name	4-Nitrophenylphosphorylcholine
CAS No.	21064-69-7
Molecular Formula	C11H17N2O7P
Molecular Weight	320.24 g/mol
Synonyms	p-Nitrophenylphosphorylcholine; Phosphorylcholine 4-nitrophenyl ester; Choline phosphate 4-nitrophenyl ester; 4-NPPC; (4-Nitrophenyl) 2-(trimethylammonio)ethyl phosphate; p-Nitrophenylphosphoryl choline; 2-[[(4-Nitrophenoxy)-hydroxyphosphoryl]oxy]-N,N,N-trimethylethanaminium
EINECS	Contact for details

Quality Control

Our 4-Nitrophenylphosphorylcholine is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our global clientele in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Solubility	Soluble in water, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.