Description

Gly-Phe p-Nitroanilide is a specialized chromogenic peptide substrate used extensively in biochemical research and diagnostic applications. This compound is critical for the specific detection and kinetic analysis of protease enzyme activity, particularly dipeptidyl peptidase IV (DPP-IV) and related enzymes. It serves as an essential tool for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require high-purity, reliable substrates for enzyme assays and diagnostic kit development.

Application

• Protease Enzyme Assays: Primary substrate for the spectrophotometric and fluorometric determination of DPP-IV (CD26) activity in research and drug discovery.
• Diagnostic Reagent Manufacturing: Key component in the formulation of clinical diagnostic kits for measuring serum protease levels.
• Biochemical Research: Used in kinetic studies, enzyme inhibition screening, and mechanistic investigations of peptidase function.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize novel DPP-IV inhibitors for diabetes and other therapeutic areas.
• Quality Control in Bioprocessing: Acts as a process control reagent to monitor protease activity during the production of biopharmaceuticals.
• Academic & Institutional Laboratories: Fundamental reagent for teaching and advanced research in enzymology and cell biology.

Basic Information

Product Name	Gly-Phe p-Nitroanilide
CAS No.	21027-72-5
Molecular Formula	C17H16N4O5
Molecular Weight	356.34 g/mol
Synonyms	Glycyl-L-phenylalanine 4-nitroanilide; Gly-Phe-pNA; H-Gly-Phe-pNA; DPP-IV Substrate; N-(3-Carboxy-1-oxopropyl)-L-phenylalanine 4-nitroanilide; L-Phenylalanine, N-(3-carboxy-1-oxopropyl)-, 4-nitroanilide; GPpNA; Dipeptidyl Peptidase IV Chromogenic Substrate
EINECS	Contact for details

Quality Control

Our Gly-Phe p-Nitroanilide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We support compliance with relevant GMP and ISO guidelines for research and diagnostic-grade chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity (Enzymatic)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.