Description

20-Hydroxycortisol is a significant endogenous steroid metabolite of cortisol, playing a crucial role in the study of glucocorticoid metabolism and related endocrine pathways. This high-purity reference standard is essential for researchers and manufacturers requiring precise analytical and biochemical applications. It is primarily utilized in pharmaceutical R&D, clinical diagnostics, and as a critical intermediate in the synthesis of specialized steroid compounds for the global life sciences market.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the quantitative analysis of cortisol metabolites in drug development and bioanalytical studies.
• Clinical Research & Diagnostics: Serves as a key biomarker in research assays investigating disorders of the hypothalamic-pituitary-adrenal (HPA) axis, Cushing's syndrome, and adrenal function.
• Biochemical Research: Employed in enzymatic studies to investigate the 11β-hydroxysteroid dehydrogenase (11β-HSD) pathways and glucocorticoid metabolism.
• Analytical Chemistry: Acts as a critical standard for method development and validation in techniques like LC-MS/MS, HPLC, and GC-MS for steroid profiling.
• Intermediate for Synthesis: Used in the preparation of more complex steroid derivatives and labeled compounds for tracer studies.

Basic Information

Product Name	20-Hydroxycortisol
CAS No.	2899-95-8
Molecular Formula	C₂₁H₃₀O₆
Molecular Weight	378.46 g/mol
Synonyms	20-Hydroxycortisol; 11β,17α,20β,21-Tetrahydroxypregn-4-ene-3-one; 20β-Hydroxycortisol; 20β-Hydroxyhydrocortisone; 20-Dihydrocortisol; Cortisol, 20-hydroxy-; Reichstein's Substance V; 20β,21-Dihydroxy-4-pregnene-3,11,20-trione
EINECS	220-798-8

Quality Control

Every batch of our 20-Hydroxycortisol is manufactured and tested under a strict quality management system. We provide comprehensive analytical data to ensure identity, purity, and consistency, meeting the stringent requirements for research and development. A detailed Certificate of Analysis (COA) is supplied with each shipment, documenting results from HPLC, NMR, and MS analyses.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or refrigerated (2-8°C) for long-term stability. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept in a dry environment and sealed after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.