Description

2-Nitrophenyl-β-D-Glucopyranoside is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of β-glucosidase activity, a key enzyme in various biological and industrial processes. It is primarily utilized by researchers and manufacturers in the life sciences, diagnostic development, and enzyme production industries for precise analytical measurements.

Application

• Enzymatic Substrate for β-Glucosidase: Serves as a key chromogenic reagent for the spectrophotometric detection and kinetic study of β-glucosidase enzymes.
• Diagnostic Kit Development: Used in the formulation of clinical and research diagnostic kits for enzyme deficiency disorders, such as Gaucher disease.
• Biochemical Research: Employed in laboratory research to investigate enzyme mechanisms, inhibition, and activity in cell lysates and purified preparations.
• Microbiology and Cell Biology: Applied as a substrate in assays to study microbial metabolism and gene expression linked to glucosidase activity.
• Quality Control in Enzyme Production: Utilized by manufacturers to standardize and verify the activity of commercial β-glucosidase preparations.
• Plant Biology Studies: Used in research to analyze β-glucosidase activity involved in plant defense mechanisms and metabolic pathways.

Basic Information

Product Name	2-Nitrophenyl-β-D-Glucopyranoside
CAS No.	2816-24-2
Molecular Formula	C₁₂H₁₅NO₈
Molecular Weight	301.25 g/mol
Synonyms	2-Nitrophenyl β-D-Glucopyranoside; o-Nitrophenyl-β-D-glucopyranoside; ONPG; 2-Nitrophenyl β-D-Glucoside; β-ONPG; o-Nitrophenyl-β-D-glucoside; 4-Nitrophenyl β-D-glucopyranoside (isomer); 2-Nitrophenyl-1-thio-β-D-glucopyranoside (related thio analog)
EINECS	220-571-2

Quality Control

Our 2-Nitrophenyl-β-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	158 - 162 °C
Specific Rotation	-60° to -65° (c=1 in H₂O)
Solubility	Soluble in water, DMSO; slightly soluble in methanol
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.