Description

Trypsin Nb Sequencing Grade From Porcine Pancreas is a highly purified proteolytic enzyme specifically processed for demanding molecular biology applications. Its primary value lies in its exceptional purity and low autolysis, ensuring consistent, high-fidelity results in sensitive protein sequencing and peptide mapping workflows. This product is essential for researchers and manufacturers in the biopharmaceutical, proteomics, and diagnostic reagent development sectors who require reliable enzymatic tools for structural analysis.

Application

• Protein Sequencing: Primary enzyme for the digestion of proteins into peptides for Edman degradation or mass spectrometry analysis.
• Peptide Mapping: Used to generate reproducible peptide fragments for the characterization and identification of proteins.
• Mass Spectrometry Sample Prep: Digests proteins for bottom-up proteomics workflows, including LC-MS/MS.
• Cell Culture & Bioprocessing: For the gentle detachment of adherent mammalian cells without damaging cell surface proteins.
• Diagnostic Manufacturing: A key component in the production of certain diagnostic kits and biochemical assays.
• Structural Biology: Limited proteolysis experiments to study protein domain structure and folding.

Basic Information

Product Name	Trypsin Nb Sequencing Grade From Porcine Pancreas
CAS No.	2594-14-1
Molecular Formula	Contact for details
Molecular Weight	~23.8 kDa (for trypsinogen)
Synonyms	Trypsin; Porcine Trypsin; Trypsin 1:250; Parenzyme; Tryptar; Trypture; Parenzymol; Protease, Trypsin; EC 3.4.21.4
EINECS	220-001-5

Quality Control

Our Sequencing Grade Trypsin is manufactured under stringent conditions to ensure it meets the high-purity requirements for research and analytical applications. Each lot is tested for specific activity, absence of contaminating proteases (e.g., chymotrypsin), and low autolysis profile. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and functional testing is provided with every shipment to guarantee batch-to-batch consistency and performance.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can lead to loss of activity and stability.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Specific Activity	> 10,000 BAEE units/mg protein
Protein Content (Biuret)	> 90%
Chymotrypsin Activity	< 0.1 BAEE units/mg trypsin
Loss on Drying	< 5.0 %
Solubility	Clear, colorless solution in 1mM HCl

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.