Description

D-Luciferin is a high-purity, light-emitting substrate essential for bioluminescence reactions. This compound is the critical reagent for the firefly luciferase enzyme, enabling sensitive detection and quantification in a wide range of assays. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical, and biotechnology industries for applications such as in vivo imaging, ATP detection, and high-throughput screening.

Application

• Bioluminescence Imaging (BLI): A vital substrate for non-invasive, real-time monitoring of tumor growth, gene expression, and cell trafficking in live animal models.
• ATP Detection & Cell Viability Assays: Used in kits and assays to measure cellular ATP levels as an indicator of cell viability, cytotoxicity, and metabolic activity.
• Reporter Gene Assays: Enables the study of gene regulation and promoter activity when coupled with the luciferase reporter gene in molecular biology research.
• High-Throughput Screening (HTS): Serves as a key component in drug discovery platforms for identifying potential therapeutic compounds that modulate biological pathways.
• Microbial Detection: Applied in systems for the rapid detection of microbial contamination by targeting microbial ATP.
• Environmental Monitoring: Used in biosensors to detect specific pollutants or toxicants based on their inhibitory effects on bioluminescent organisms or engineered cells.

Basic Information

Product Name	D-Luciferin
CAS No.	2591-17-5
Molecular Formula	C11H8N2O3S2
Molecular Weight	280.32 g/mol
Synonyms	(-)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid; D-(-)-Luciferin; Firefly Luciferin; Beetle Luciferin; (4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid; LH2; Luciferin, firefly
EINECS	219-991-7

Quality Control

Our D-Luciferin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescence Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.