Description

4-Nitrophenyl-β-D-Glucopyranoside is a chromogenic substrate widely used in enzymatic assays. This compound matters for its critical role in the detection and quantification of β-glucosidase and cellulase enzyme activity, providing a reliable colorimetric readout. Industries that need it include biotechnology, pharmaceutical research, and diagnostic manufacturing for quality control and biochemical research applications.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of β-glucosidase activity in research and industrial enzyme characterization.
• Diagnostic Kits: Used as a key reagent in clinical and biochemical diagnostic kits for detecting specific enzymatic markers.
• Cellulase Research: Essential for studying cellulase enzyme systems and evaluating the efficiency of cellulolytic processes in biofuel and textile industries.
• Microbiology: Employed in microbial identification and studies of bacterial and fungal metabolism that involve glycoside hydrolases.
• Pharmaceutical QC: Utilized in quality control laboratories to test enzyme-based pharmaceuticals and raw materials.
• Biochemical Research: Serves as a standard tool in academic and industrial research for inhibitor studies and enzyme kinetics.

Basic Information

Product Name	4-Nitrophenyl-β-D-Glucopyranoside
CAS No.	2492-87-7
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	p-Nitrophenyl β-D-glucopyranoside; p-Nitrophenyl β-D-glucoside; 4-Nitrophenyl β-D-glucoside; PNP-G; PNP-β-D-glucopyranoside; β-4-Nitrophenyl glucoside; 4-NPG; 1-O-(4-Nitrophenyl)-β-D-glucopyranoside
EINECS	219-672-2

Quality Control

Our 4-Nitrophenyl-β-D-Glucopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets high-purity standards suitable for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Approx. 195-200°C (dec.)
Specific Rotation	-60° to -65° (c=1 in H2O)
Solubility	Soluble in water, DMSO; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.