Description

n-Succinyl-L-Phenylalanine p-Nitroanilide is a specialized chromogenic substrate designed for enzymatic assays. This compound matters for its high specificity and sensitivity in detecting and quantifying protease activity, particularly from enzymes like chymotrypsin. It is primarily needed by research institutions, pharmaceutical developers, and diagnostic kit manufacturers engaged in biochemical research, drug discovery, and quality control processes.

Application

• Protease Activity Assays: As a selective substrate for the kinetic study and quantification of chymotrypsin and related serine proteases.
• Biochemical Research: Used in laboratory investigations of enzyme kinetics, inhibition studies, and mechanism-of-action analysis.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitor drug candidates.
• Diagnostic Reagent Formulation: A key component in the development of clinical diagnostic tests and kits for measuring protease levels.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity and purity of enzyme preparations during production.

Basic Information

Product Name	n-Succinyl-L-Phenylalanine p-Nitroanilide
CAS No.	2440-62-2
Molecular Formula	C19H19N3O6
Molecular Weight	385.37 g/mol
Synonyms	Suc-Phe-pNA; Succinyl-L-Phenylalanine p-Nitroanilide; N-Succinyl-L-Phenylalanine 4-Nitroanilide; Suc-F-pNA; Chymotrypsin Substrate Suc-Phe-pNA; Protease Substrate; 4-Nitroanilide of N-Succinyl-L-phenylalanine; N-Succinylphenylalanine p-nitroanilide
EINECS	Contact for details

Quality Control

Our n-Succinyl-L-Phenylalanine p-Nitroanilide is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.