Description

Thymidine 5'-4-Nitrophenyl Phosphate is a chemically modified nucleotide derivative where a 4-nitrophenyl group is attached to the phosphate moiety. This compound is a critical substrate and intermediate in biochemical research, particularly for studying enzyme kinetics and nucleic acid metabolism. It is essential for researchers and manufacturers in the fields of molecular biology, enzymology, and pharmaceutical development, serving as a key reagent for phosphatase and kinase assays.

Application

• Enzyme Substrate: Primarily used as a chromogenic substrate for the assay of alkaline phosphatase and other phosphomonoesterase activities, where the release of 4-nitrophenol is monitored spectrophotometrically.
• Biochemical Research: Serves as a model compound for studying phosphorylation and dephosphorylation mechanisms in nucleotide chemistry and signal transduction pathways.
• Diagnostic Reagent Manufacturing: Employed in the formulation of diagnostic kits for clinical and research laboratories to measure enzyme levels.
• Pharmaceutical Intermediate: Acts as a key synthetic building block in the development of novel nucleotide-based prodrugs and antiviral agents.
• Enzyme Inhibitor Studies: Utilized in high-throughput screening (HTS) assays to identify and characterize potential inhibitors of nucleotide-processing enzymes.

Basic Information

Product Name	Thymidine 5'-4-Nitrophenyl Phosphate
CAS No.	2304-08-7
Molecular Formula	C16H19N3O9P
Molecular Weight	428.31 g/mol
Synonyms	4-Nitrophenyl Thymidine 5'-Monophosphate; p-Nitrophenyl Thymidine 5'-Phosphate; Thymidine 5'-(4-nitrophenyl phosphate); 5'-Thymidylic Acid 4-Nitrophenyl Ester; PNPT; 4-Nitrophenyl TMP; Thymidine 5'-Monophosphate p-Nitrophenyl Ester; 2'-Deoxythymidine 5'-(4-nitrophenyl phosphate)
EINECS	Contact for details

Quality Control

Our Thymidine 5'-4-Nitrophenyl Phosphate is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment to maintain stability and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.