Description

25-Hydroxycholesterol is a significant oxysterol metabolite of cholesterol, playing a crucial role in lipid metabolism and cell signaling pathways. Its importance stems from its function as a potent endogenous regulator of cholesterol homeostasis and as a ligand for liver X receptors (LXRs). This high-purity biochemical is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors, particularly for studies in cholesterol metabolism, atherosclerosis, and as a reference standard in analytical applications. CAS No. 2140-46-7.

Application

• Biochemical Research: A key reference standard and reagent for studying cholesterol biosynthesis, transport, and metabolism.
• Pharmaceutical Development: Used in drug discovery programs targeting liver X receptors (LXRs) for metabolic and inflammatory diseases.
• Cell Biology Studies: Investigated for its roles in cell proliferation, differentiation, and immune modulation.
• Analytical Standard: Serves as a critical calibration standard in HPLC, LC-MS, and GC-MS methods for oxysterol analysis.
• Metabolic Pathway Analysis: Essential for research into atherosclerosis, neurodegenerative diseases, and cancer biology.
• In Vitro Diagnostics (IVD): Potential component in assay kits for cholesterol-related metabolic profiling.

Basic Information

Product Name	25-Hydroxycholesterol
CAS No.	2140-46-7
Molecular Formula	C27H46O2
Molecular Weight	402.66 g/mol
Synonyms	(25R)-Cholest-5-ene-3β,25-diol; 25-Hydroxycholest-5-en-3β-ol; 3β,25-Dihydroxycholest-5-ene; Cholest-5-ene-3β,25-diol; 25-HC; 25-OHC; 25-Hydroxy Cholesterol; NSC 82287
EINECS	218-376-9

Quality Control

Our 25-Hydroxycholesterol is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and development. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines to support your regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. The product is light-sensitive and easily oxidized. Allow the sealed vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Solubility	Soluble in ethanol, DMSO; slightly soluble in chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.