Description

4-Nitrophenyl-β-D-Xylopyranoside is a chromogenic synthetic substrate specifically designed for enzymatic assays. This compound is critical for the precise detection and quantification of β-xylosidase activity, a key enzyme in various biochemical pathways. It is an essential reagent for researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic industries, where accurate enzyme analysis is paramount for product development and process validation.

Application

• Enzymatic Assay Substrate: Primary substrate for the colorimetric determination of β-xylosidase (xylan 1,4-β-xylosidase) activity in research and industrial applications.
• Biochemical Research: Used in studies of carbohydrate-active enzymes (CAZymes), hemicellulose degradation pathways, and enzyme kinetics.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for measuring specific enzyme levels in clinical and agricultural samples.
• Quality Control in Biofuel Production: Employed to monitor and optimize the activity of xylose-releasing enzymes in lignocellulosic biomass processing for biofuel and biorefinery operations.
• Food & Beverage Industry: Used in enzyme activity assays to control processes in fruit juice clarification, brewing, and baking where hemicellulose breakdown is involved.
• Pharmaceutical Development: Serves as a tool enzyme substrate in drug discovery programs targeting carbohydrate metabolism.

Basic Information

Product Name	4-Nitrophenyl-β-D-Xylopyranoside
CAS No.	2001-96-9
Molecular Formula	C11H13NO7
Molecular Weight	271.22 g/mol
Synonyms	p-Nitrophenyl β-D-xylopyranoside; 4-Nitrophenyl β-D-xyloside; PNPG-Xyl; pNP-β-D-Xyl; β-4-Nitrophenyl xylopyranoside; 4-Nitrophenyl-β-xyloside; 1-O-(4-Nitrophenyl)-β-D-xylopyranose; p-Nitrophenyl-β-D-xylopyranoside
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-β-D-Xylopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and performance consistency suitable for sensitive enzymatic assays. Certificates of Analysis (COA) detailing purity, identity, and specific performance data are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF
Enzymatic Suitability	Passes performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.