Description

H-Met-Leu-Amc Tfa is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of enzymatic activity. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical industries for high-throughput screening and mechanistic studies.

Application

• Protease Enzyme Assays: As a fluorogenic substrate for measuring the activity of specific proteases, including aminopeptidases and related enzymes.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential protease inhibitors.
• Biochemical Research: A key tool for studying enzyme kinetics, substrate specificity, and catalytic mechanisms in vitro.
• Diagnostic Development: Employed in the research and development of clinical diagnostic kits that measure protease levels.
• Pharmaceutical QC: Applied in quality control processes to ensure the consistency and potency of enzyme-based biopharmaceuticals.

Basic Information

Item	Detail
Product Name	H-Met-Leu-Amc Tfa
CAS No.	1926163-55-4
Molecular Formula	C22H30F3N5O6S
Molecular Weight	549.56 g/mol
Synonyms	H-Met-Leu-AMC TFA; H-Met-Leu-AMC trifluoroacetate; Methionyl-leucyl-7-amido-4-methylcoumarin TFA salt; L-Methionyl-L-leucine 7-amido-4-methylcoumarin TFA; Fluorogenic substrate MCA; Met-Leu-AMC TFA; Protease substrate 1926163-55-4; Aminopeptidase substrate
EINECS	Contact for details

Quality Control

Our H-Met-Leu-Amc Tfa is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant standards for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.