Description

Z-Asp-Gln-Met-Asp-Afc CAS NO 1926163-45-2 is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound matters as a critical research tool, enabling precise and sensitive measurement of caspase and other protease enzyme activity in biochemical and cell-based studies. Life science researchers and pharmaceutical developers need it for applications in apoptosis research, drug discovery screening, and enzymatic pathway analysis.

Application

• Caspase Activity Assays: Primary substrate for fluorometric detection of caspase-3 and related protease activity in apoptosis studies.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors or activators.
• Biochemical Research: Essential reagent for studying protease kinetics, specificity, and enzymatic mechanisms in purified systems.
• Cell Biology Studies: Employed in cell lysates or live-cell imaging to monitor protease activation during cellular processes like programmed cell death.
• Diagnostic Development: Serves as a key component in developing diagnostic assays for diseases involving dysregulated protease activity.
• Quality Control in Biologics: Used to assess the presence and activity of specific proteases in biomanufacturing processes.

Basic Information

Product Name	Z-Asp-Gln-Met-Asp-Afc
CAS No.	1926163-45-2
Molecular Formula	C33H41N7O12S
Molecular Weight	759.78 g/mol
Synonyms	Z-DEVD-AFC; Z-Asp(OMe)-Gln-Met-Asp(OMe)-AFC; Ac-DEVD-AFC; Caspase-3 Substrate IV; Ac-Asp-Glu-Val-Asp-7-amino-4-trifluoromethylcoumarin; Z-DEVD-AFC Caspase Substrate; AFC-labeled DEVD peptide; Fluorogenic Caspase-3/7 Substrate
EINECS	Contact for details

Quality Control

Our Z-Asp-Gln-Met-Asp-Afc is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic confirmation of structure. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF
Functional Activity	Conforms to reference standard in caspase-3 assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.