Description

H-Glu(Edans)-Lys-Pro-Ala-Lys-Phe-Phe-Arg-Leu-Lys(Dabcyl)-Nh2 is a specialized, high-purity synthetic peptide substrate designed for sensitive biochemical assays. This compound matters because it serves as a critical tool for the specific detection and quantification of protease activity, particularly for enzymes like furin. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors who require reliable, high-performance reagents for drug discovery, enzymatic research, and diagnostic development.

Application

• Protease Activity Assays: Primary substrate for the fluorometric detection and kinetic analysis of furin and related proprotein convertases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: A key reagent in studying protease specificity, enzyme kinetics, and proteolytic signaling pathways in cellular processes.
• Diagnostic Development: Employed in the development of fluorescent-based diagnostic kits and assays for detecting specific protease biomarkers.
• Quality Control in Biologics: Used to monitor and ensure the activity and consistency of enzyme preparations used in biomanufacturing.
• Virology & Infectious Disease Research: Applied in studies of viral entry and pathogenesis, as many viruses utilize host proteases like furin for activation.

Basic Information

Product Name	H-Glu(Edans)-Lys-Pro-Ala-Lys-Phe-Phe-Arg-Leu-Lys(Dabcyl)-Nh2
CAS No.	1926163-42-9
Molecular Formula	C91H124N22O22
Molecular Weight	~1830.1 g/mol
Synonyms	Edans-FRET Substrate for Furin; Dabcyl-Edans FRET Peptide; Fluorogenic Furin Substrate; H-Glu(Edans)-Lys-Pro-Ala-Lys-Phe-Phe-Arg-Leu-Lys(Dabcyl)-Amide; 5-((2-Aminoethyl)amino)naphthalene-1-sulfonyl (Edans) and 4-((4-(Dimethylamino)phenyl)azo)benzoic acid (Dabcyl) labeled peptide; FRET Peptide Substrate 1926163-42-9; Mca-PLGL-Dpa-AR-NH2 analog for furin; Proprotein Convertase Substrate.
EINECS	Contact for details

Quality Control

Every batch of our synthetic peptide is manufactured under strict cGMP-like conditions and undergoes comprehensive analytical characterization to ensure identity, purity, and performance. Quality is verified by HPLC, MS (Mass Spectrometry), and amino acid analysis. A detailed Certificate of Analysis (COA) providing lot-specific data for purity, water content, and peptide content is supplied with each shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with theoretical molecular weight
Purity (HPLC)	≥ 95% (Area Percent)
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Cleavage by furin produces characteristic fluorescence increase (Contact for specific δF/min/µg data)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.