Description

Abz-Ala-Phe-Arg-Phe-Ser-Gln-Eddnp is a specialized, sequence-defined peptide derivative containing a fluorescent 2,4-dinitrophenyl (Eddnp) quencher group. This compound matters as a critical research tool for studying protease activity and enzyme kinetics, offering high sensitivity and specificity in fluorescence resonance energy transfer (FRET)-based assays. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic developers focused on cardiovascular, neurological, and oncological research.

Application

• Protease Substrate & Enzyme Assays: Acts as a specific, fluorogenic substrate for the quantitative measurement of thrombin, trypsin, and other serine protease activities in high-throughput screening (HTS).
• Biochemical Research & Mechanism Studies: Used in fundamental research to investigate enzyme inhibition, catalytic mechanisms, and substrate specificity of various proteases.
• Drug Discovery & Development: Employed in pharmaceutical R&D for screening and characterizing potential therapeutic protease inhibitors for conditions like thrombosis, inflammation, and cancer.
• Diagnostic Kit Development: Serves as a key component in the development of sensitive in vitro diagnostic (IVD) kits for clinical biomarker detection related to coagulation disorders.
• Cell Biology & Signal Transduction: Utilized to monitor and visualize protease activity in live-cell imaging and studies of cellular signaling pathways.

Basic Information

Item	Detail
Product Name	Abz-Ala-Phe-Arg-Phe-Ser-Gln-Eddnp
CAS No.	1926163-28-1
Molecular Formula	C58H75N17O17
Molecular Weight	1250.3 g/mol
Synonyms	Abz-AFRFSQ-Eddnp; FRET Substrate for Thrombin; o-Aminobenzoic acid-Ala-Phe-Arg-Phe-Ser-Gln-N-(2,4-Dinitrophenyl)ethylenediamine; Fluorogenic Peptide Substrate Abz-AFRFSQ-Eddnp; Thrombin Substrate III (FRET-based); EDANS/DABCYL FRET Peptide; Protease Fluorescence Quencher Substrate
EINECS	Contact for details

Quality Control

Every batch of Abz-Ala-Phe-Arg-Phe-Ser-Gln-Eddnp is manufactured and tested under strict quality management systems. We guarantee high-purity material suitable for sensitive research and assay development. Comprehensive analytical data, including HPLC purity, MS confirmation, and functional activity validation, is provided with each shipment. Certificates of Analysis (COA) are available upon request and are essential for regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Validated in a standard thrombin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.