Description

Tco4-Peg3-Maleimide CAS NO 1914971-04-2 is a specialized heterobifunctional crosslinker designed for advanced bioconjugation and chemical biology applications. This compound integrates a trans-cyclooctene (TCO) group for rapid, bioorthogonal tetrazine ligation with a maleimide moiety for selective thiol coupling, connected by a triethylene glycol (PEG3) spacer to enhance solubility and reduce steric hindrance. It is an essential reagent for researchers and developers in the pharmaceutical and diagnostic sectors who require precise, modular, and efficient methods for labeling proteins, antibodies, and nanoparticles. Its design facilitates the creation of stable, well-defined conjugates critical for next-generation therapeutics and imaging agents.

Application

• Antibody-Drug Conjugate (ADC) Payload Attachment: Site-specific conjugation of cytotoxic drugs or imaging probes to monoclonal antibodies via thiol-maleimide chemistry, followed by TCO-tetrazine click chemistry for modular assembly.
• Protein and Peptide Labeling: Functionalization of cysteine-containing biomolecules with TCO handles for subsequent labeling with tetrazine-modified fluorophores, affinity tags, or other functional groups.
• Surface Functionalization of Nanoparticles: Modification of gold, silica, or polymeric nanoparticle surfaces to introduce bioorthogonal reactivity for targeted drug delivery or diagnostic applications.
• Pretargeted Imaging Strategies: A key component in multistep pretargeting protocols for in vivo imaging (e.g., PET, SPECT), where the TCO group is attached to a targeting vector and later reacted with a radiolabeled tetrazine.
• Hydrogel and Biomaterial Crosslinking: Incorporation into polymer networks to create chemically defined, stimuli-responsive biomaterials for 3D cell culture and tissue engineering.
• Diagnostic Assay Development: Construction of sensitive immunoassays by conjugating detection enzymes or signal-amplifying molecules to antibodies or antigens.

Basic Information

Product Name	Tco4-Peg3-Maleimide
CAS No.	1914971-04-2
Molecular Formula	C₂₃H₃₃N₃O₇
Molecular Weight	463.53 g/mol
Synonyms	trans-Cyclooctene-PEG3-Maleimide; TCO-PEG3-MAL; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-oxo-2,5,8,11-tetraoxatetradecan-14-yl) carbamate; TCO-(PEG)3-Maleimide; 2,5,8,11-Tetraoxatetradecan-14-oic acid, 14-[[(1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethoxy]carbonyl]amino]-4-oxo-, 2,5-dioxo-1-pyrrolidinyl ester; TCO-PEG3-NHS-Maleimide (common naming convention); TCO-PEG(3)-MAL
EINECS	Contact for details

Quality Control

Every batch of Tco4-Peg3-Maleimide is manufactured under strict quality management systems and undergoes comprehensive analytical characterization to ensure identity, purity, and performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, NMR (¹H and ¹³C), and mass spectrometry analysis. Our quality commitment ensures the reagent meets the high standards required for sensitive bioconjugation work in research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.