Description

Diazo Biotin-Peg3-Alkyne is a multifunctional, heterobifunctional crosslinking reagent designed for advanced bioconjugation and chemical biology research. This compound integrates a diazirine photoactivatable group, a biotin affinity tag, and a terminal alkyne for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry, enabling precise, multi-step labeling strategies. It is essential for researchers in proteomics, target identification, and biomaterial surface functionalization who require robust, site-specific tagging of biomolecules under mild conditions. The product is supplied as a high-purity building block for creating innovative probes and diagnostic tools.

Application

• Photoaffinity Labeling (PAL): The diazirine group generates highly reactive carbenes upon UV irradiation (≈365 nm) for covalent crosslinking to proteins, nucleic acids, or other biomolecules of interest, facilitating the study of transient molecular interactions.
• Activity-Based Protein Profiling (ABPP): Used to design probes that covalently bind to active enzyme sites, followed by enrichment via the biotin tag and analysis by mass spectrometry for drug discovery and target validation.
• Chemical Proteomics & Target Identification: Enables the identification of unknown protein targets for small molecule drugs or natural products through photo-crosslinking, biotin-based pull-down, and streptavidin affinity purification.
• Biomaterial & Surface Engineering: The terminal alkyne allows for subsequent click chemistry modifications, making it valuable for functionalizing nanoparticles, hydrogels, or biosensor surfaces with bioactive molecules.
• DNA/RNA Interactome Mapping: Employed in developing probes to capture proteins that interact with specific nucleic acid sequences or structures, aiding in epigenetics and transcriptional regulation studies.
• Development of Diagnostic Reagents: Serves as a key intermediate in synthesizing advanced diagnostic conjugates for ELISA, lateral flow assays, or imaging, leveraging the strong biotin-streptavidin interaction for signal amplification.

Basic Information

Product Name	Diazo Biotin-Peg3-Alkyne
CAS No.	1884349-58-9
Molecular Formula	C₂₈H₄₄N₆O₇S
Molecular Weight	608.8 g/mol
Synonyms	Biotin-PEG3-Diazirine-Alkyne; Alkyne-PEG3-Biotin-Diazirine; Diazirine-Biotin-PEG3-Propargyl; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(3-(2-(3-(3-(prop-2-yn-1-yloxy)propanamido)propanamido)propanamido)propyl)pentanamide-4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzamide; Photoactivatable Biotin Alkyne; Heterobifunctional Biotin Crosslinker; Biotinylated Diazirine Alkyne Probe
EINECS	Contact for details

Quality Control

Our Diazo Biotin-Peg3-Alkyne is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment. We support custom synthesis and can tailor specifications to meet specific project requirements for advanced probe development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the primary container sealed in a secondary container with an appropriate desiccant. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.