Description

3-Deaza-2’-Deoxyuridine-5''-Triphosphate is a chemically modified deoxyribonucleotide triphosphate (dNTP) analog, specifically engineered for advanced molecular biology and biochemical research. This compound is valued for its ability to act as a substrate or inhibitor for DNA polymerases and other nucleotide-processing enzymes, enabling studies on enzyme mechanisms, nucleic acid synthesis, and antiviral drug development. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical R&D departments focused on virology, enzymology, and nucleotide metabolism.

Application

• Enzyme Mechanism Studies: Used as a substrate analog to investigate the specificity and catalytic mechanisms of DNA polymerases, reverse transcriptases, and other nucleic acid polymerases.
• Antiviral Research: Serves as a potential chain terminator or inhibitor in the development of nucleoside analog-based antiviral therapies.
• PCR and Molecular Diagnostics: Can be incorporated into specialized PCR assays or probe synthesis to study mutation detection or to create modified nucleic acid templates.
• Biochemical Assay Development: Employed in the development of high-throughput screening assays for enzyme activity and inhibitor discovery.
• Nucleotide Metabolism Research: Acts as a tool compound to study pathways involving nucleotide salvage and incorporation.
• Structural Biology: Used in crystallography or NMR studies to understand enzyme-substrate interactions at the atomic level.

Basic Information

Product Name	3-Deaza-2’-Deoxyuridine-5''-Triphosphate
CAS No.	1881279-84-0
Molecular Formula	C11H17N2O13P3
Molecular Weight	502.18 g/mol
Synonyms	3-Deaza-dUTP; 3-Deaza-2'-deoxyuridine 5'-triphosphate; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-1,4-dihydro-4-oxo-3-pyridinecarboxylic acid 5'-(trihydrogen triphosphate); 3-Deaza-2'-deoxyuridine-5'-triphosphate trisodium salt (common salt form); 3-Deaza-dU 5'-triphosphate; Modified dNTP analog; 3-Deaza-2'-deoxyuridine triphosphate
EINECS	Contact for details

Quality Control

Our 3-Deaza-2’-Deoxyuridine-5''-Triphosphate is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0 %
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.