Description

Azadibenzocyclooctyne-Peg4-Acid is a bifunctional chemical linker featuring a highly reactive azadibenzocyclooctyne (ADIBO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry, conjugated to a hydrophilic tetraethylene glycol (PEG4) spacer and a terminal carboxylic acid. This compound matters for its exceptional bioorthogonal reactivity, enabling rapid, catalyst-free, and high-yield conjugation of biomolecules under mild, physiological conditions without interfering with native biological processes. It is essential for researchers and manufacturers in bioconjugation, proteomics, drug discovery, and the development of antibody-drug conjugates (ADCs) and diagnostic probes who require efficient and selective labeling strategies.

Application

• Bioconjugation and Protein Labeling: For site-specific modification of antibodies, peptides, and other proteins via reaction with azide-functionalized targets.
• Antibody-Drug Conjugate (ADC) Development: As a key linker component for attaching cytotoxic payloads to monoclonal antibodies through bioorthogonal chemistry.
• Proteomics and Biomarker Discovery: To tag and enrich azide-modified biomolecules for mass spectrometry analysis and cellular imaging.
• Polymer and Material Science: For surface functionalization of nanoparticles, hydrogels, and other materials to introduce carboxylic acid groups for further coupling.
• Diagnostic Probe Synthesis: In the construction of fluorescent, radioactive, or other reporter molecules for in vitro and in vivo diagnostics.
• Chemical Biology Tool Development: As a versatile reagent for creating activity-based probes and studying biomolecular interactions in live cells.

Basic Information

Product Name	Azadibenzocyclooctyne-Peg4-Acid
CAS No.	1870899-46-9
Molecular Formula	C31H38N2O8
Molecular Weight	566.65 g/mol
Synonyms	ADIBO-PEG4-COOH; DBCO-PEG4-Acid; Azadibenzocyclooctyne-PEG4-carboxylic acid; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (2-{2-[2-(2-{2-[(2,5-dioxopyrrolidin-1-yl)oxy]-2-oxoethoxy}ethoxy)ethoxy]ethoxy})acetate; SPAAC Reagent PEG4 Acid; DBCO-PEG4-CO2H; BCN-PEG4-Acid (Note: BCN is a related but distinct cyclooctyne); 1,3-Dipolar Cycloaddition Reagent
EINECS	Contact for details

Quality Control

Our Azadibenzocyclooctyne-Peg4-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive research and development applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms (M+Na+ or M+H+)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.