Description

6-Chloro-4-Methylumbelliferyl β-Lactoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of enzymatic activity. This compound matters because it offers high specificity and a strong fluorescent signal upon enzymatic cleavage, enabling precise biochemical and diagnostic assays. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for applications such as high-throughput screening and enzyme kinetics studies.

Application

• Enzyme Assay Substrate: A critical reagent for the detection and kinetic analysis of β-galactosidase and related glycosidase enzymes in research and diagnostic kits.
• High-Throughput Screening (HTS): Used in pharmaceutical and biotechnology research for screening compound libraries against enzymatic targets due to its fluorogenic properties.
• Cell-Based Reporter Assays: Employed in molecular biology to monitor gene expression and promoter activity when linked to a β-galactosidase reporter gene.
• Diagnostic Development: Serves as a key component in the development of clinical diagnostic tests for enzymatic deficiencies or bacterial identification (e.g., E. coli).
• Biochemical Research: Utilized in fundamental research to study enzyme mechanisms, inhibition, and substrate specificity.
• Quality Control in Biomanufacturing: Applied in-process testing to ensure the presence and activity of specific enzymes during production.

Basic Information

Product Name	6-Chloro-4-Methylumbelliferyl β-Lactoside
CAS No.	1867162-15-9
Molecular Formula	C20H23ClO12
Molecular Weight	490.84 g/mol
Synonyms	6-Chloro-4-methylumbelliferyl-β-D-galactopyranoside; CMU-β-Gal; 6-Chloro-4-MU-β-Gal; 6-Chloro-4-methylumbelliferyl β-D-galactoside; β-Galactosidase substrate 6-chloro-4-methylumbelliferyl; Fluorogenic β-galactosidase substrate; 6-Cl-4-MU-β-Gal; (2R,3R,4S,5R,6R)-2-[[(2R,3S,4R,5R,6S)-6-(5-Chloro-2-oxo-2H-chromen-7-yl)oxy-3,4,5-trihydroxyoxan-2-yl]methoxy]-6-(hydroxymethyl)oxane-3,4,5-triol
EINECS	Contact for details

Quality Control

Our 6-Chloro-4-Methylumbelliferyl β-Lactoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Functional in standard β-galactosidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.