Description

6-Chloro-4-Methylumbelliferyl β-D-Galactopyranoside is a specialized fluorogenic substrate designed for the sensitive detection of β-galactosidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic quantification is required. It is primarily utilized by biotechnology firms, diagnostic kit manufacturers, and academic research laboratories in life sciences.

Application

• Biochemical Research: A key fluorogenic substrate for the assay and kinetic study of β-galactosidase activity in vitro.
• Diagnostic Assays: Used as a core component in enzymatic diagnostic kits for clinical and research purposes, including reporter gene assays.
• Cell Biology & Microbiology: Employed in staining protocols to detect β-galactosidase expression in cells (e.g., lacZ gene reporter systems) and for bacterial identification.
• High-Throughput Screening (HTS): Integral to fluorescence-based HTS platforms in drug discovery for identifying modulators of enzyme activity.
• Food Safety & Quality Control: Applied in enzymatic methods for the detection of specific microorganisms in food and environmental samples.
• Molecular Biology Reagents: A component in specialized buffers and reagent mixes for molecular biology applications requiring high sensitivity.

Basic Information

Product Name	6-Chloro-4-Methylumbelliferyl β-D-Galactopyranoside
CAS No.	1867162-06-8
Molecular Formula	C16H17ClO8
Molecular Weight	372.75 g/mol
Synonyms	6-Chloro-4-methylumbelliferyl β-D-galactopyranoside; 6-Chloro-4-MU-β-D-galactopyranoside; 6-Cl-4-MU-β-Gal; CMU-β-Gal; β-Galactosidase substrate 6-Cl-4-MU; 6-Chloro-4-methyl-2-oxo-2H-chromen-7-yl β-D-galactopyranoside; 6-Chloro-4-methylumbelliferyl-β-D-galactoside; 6-Chloro-4-methyl-7-β-galactopyranosyloxycoumarin
EINECS	Contact for details

Quality Control

Our 6-Chloro-4-Methylumbelliferyl β-D-Galactopyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorescence Properties	Excitation/Emission maxima consistent with standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.