Description

Azido-PEG3-Maleimide is a heterobifunctional crosslinker featuring a maleimide group for thiol conjugation and an azide group for copper-free click chemistry via strain-promoted alkyne-azide cycloaddition (SPAAC). This compound is essential for creating stable, site-specific bioconjugates, particularly in aqueous environments where traditional copper-catalyzed reactions are incompatible. It is a critical reagent for researchers and manufacturers in biopharmaceutical development, antibody-drug conjugate (ADC) synthesis, and advanced materials science, enabling the precise labeling and functionalization of proteins, peptides, and nanoparticles.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein and Peptide Labeling: Fluorescent or functional tag attachment for imaging, diagnostics, and tracking studies.
• Surface Functionalization: Modification of nanoparticles, biosensors, and microarray surfaces for targeted binding and assay development.
• Polymer and Hydrogel Synthesis: Crosslinking agent for creating smart biomaterials with tailored degradation and release profiles.
• Bioconjugation for Diagnostic Kits: Immobilization of enzymes or antibodies on solid supports in lateral flow and ELISA platforms.
• Chemical Biology Probes: Tool compound for studying protein-protein interactions and cellular pathways using bioorthogonal chemistry.

Basic Information

Product Name	Azido-PEG3-Maleimide
CAS No.	1858264-36-4
Molecular Formula	C15H22N4O6
Molecular Weight	354.36 g/mol
Synonyms	Azido-PEG3-Mal; Maleimide-PEG3-Azide; 3-Azidopropyl-PEG3-maleimide; N-(Azido-PEG3)-maleimide; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]propanoate; PEG3 Azide Maleimide; Azido-triethyleneglycol-maleimide
EINECS	Contact for details

Quality Control

Our Azido-PEG3-Maleimide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and mass spectrometry to ensure identity and consistency. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to guarantee the product meets the stringent requirements for research and bioconjugation applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, and aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.