Description

Fmoc-Peg6-Nhs Ester CAS NO 1818294-31-3 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and an acid-labile Fmoc-protected amine. This compound is essential for creating stable, hydrophilic spacers in bioconjugation and peptide synthesis, significantly improving solubility and reducing steric hindrance. It is a critical reagent for researchers and manufacturers in pharmaceutical development, proteomics, and the production of antibody-drug conjugates (ADCs) and PEGylated therapeutics.

Application

• Bioconjugation and Linker Chemistry: Used to covalently attach peptides, proteins, or antibodies to other molecules, surfaces, or nanoparticles via its NHS ester group, with the Fmoc group providing a protected handle for further orthogonal chemistry.
• Solid-Phase Peptide Synthesis (SPPS): Serves as a hydrophilic, flexible spacer to incorporate PEG chains into synthetic peptides, enhancing their aqueous solubility and bioavailability.
• Antibody-Drug Conjugate (ADC) Development: Facilitates the construction of cleavable or non-cleavable linkers between cytotoxic payloads and monoclonal antibodies, leveraging the PEG spacer to improve pharmacokinetics.
• PEGylation Reagent: Enables site-specific PEGylation of therapeutic proteins and peptides to modulate immunogenicity, increase circulation half-life, and improve stability.
• Surface Functionalization: Employed to modify biosensor chips, microarray slides, or magnetic beads with amine-reactive groups for immobilizing biomolecules.
• Dendrimer and Polymer Synthesis: Acts as a building block for constructing branched, multi-functional polymeric architectures with controlled release properties.
• Prodrug Synthesis: Utilized to create prodrugs where the active pharmaceutical ingredient is linked via a hydrolytically or enzymatically cleavable PEG-based spacer.

Basic Information

Product Name	Fmoc-Peg6-Nhs Ester
CAS No.	1818294-31-3
Molecular Formula	C₃₇H₅₀N₂O₁₃
Molecular Weight	742.80 g/mol
Synonyms	Fmoc-NH-PEG6-NHS Ester; Fmoc-Amino-PEG6-NHS; N-(9-Fluorenylmethoxycarbonyl)-3,6,9,12,15,18-hexaoxahenicosane-21-amine, 21-((2,5-Dioxopyrrolidin-1-yl)oxy)-21-oxo-; Fmoc-PEG6-OSu; 1-[(9H-Fluoren-9-ylmethoxy)carbonyl]-3,6,9,12,15,18-hexaoxahenicosane-21-oic Acid 2,5-Dioxopyrrolidin-1-yl Ester; Fmoc-15-amino-4,7,10,13-tetraoxapentadecanoic Acid NHS Ester; Fmoc-N-amido-PEG6-NHS
EINECS	Contact for details

Quality Control

Our Fmoc-Peg6-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided to ensure the material meets the stringent requirements for research and cGMP-grade pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.