Description

Hydroxy-Peg3-Nhs is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a hydroxyl group and an N-hydroxysuccinimide (NHS) ester. This compound is essential for creating stable amide bonds between the NHS ester and primary amines, while the hydroxyl terminus provides a versatile site for further functionalization or conjugation. It is a critical reagent for researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science, enabling the precise modification of peptides, proteins, and surfaces.

Application

• Bioconjugation and ADC Development: Used as a linker to attach cytotoxic drugs, fluorescent dyes, or other payloads to antibodies, proteins, or peptides for targeted therapies and diagnostics.
• PEGylation of Biomolecules: Facilitates the site-specific PEGylation of therapeutic proteins to enhance solubility, reduce immunogenicity, and prolong circulation half-life.
• Surface Functionalization: Employed to modify nanoparticles, biosensors, and microarray surfaces with amine-reactive groups for subsequent biomolecule immobilization.
• Hydrogel and Polymer Synthesis: Serves as a crosslinking agent or functional monomer in the synthesis of biocompatible hydrogels and smart polymeric materials.
• Diagnostic Reagent Preparation: Key component in the development of enzyme conjugates and labeled probes for immunoassays (ELISA, lateral flow) and histochemistry.
• Drug Delivery System Fabrication: Integrates into the design of micelles, liposomes, and dendrimers to create targeted and controlled-release drug delivery platforms.

Basic Information

Product Name	Hydroxy-Peg3-Nhs
CAS No.	1807518-71-3
Molecular Formula	C₁₇H₂₉NO₉
Molecular Weight	391.42 g/mol
Synonyms	3-((2-(2-(3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropoxy)ethoxy)ethoxy)propan-1-ol; 1-[3-(2-{2-[2-(3-Hydroxypropoxy)ethoxy]ethoxy}propanoyloxy)-2,5-dioxopyrrolidin-1-yl]; Hydroxy-PEG3-NHS ester; NHS-PEG3-OH; α-Hydroxy-ω-NHS-PEG3; N-Hydroxysuccinimide ester of carboxy-PEG3-alcohol; PEG3 spacer NHS ester
EINECS	Contact for details

Quality Control

Our Hydroxy-Peg3-Nhs is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for research and pharmaceutical applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.