Description

Methyltetrazine-Peg5-Alkyne is a heterobifunctional crosslinking reagent designed for bioorthogonal conjugation chemistry. This compound is essential for researchers and developers requiring precise, stable, and efficient labeling of biomolecules under mild, aqueous conditions. It is primarily used by professionals in pharmaceutical R&D, bioconjugation, and advanced materials science for creating targeted diagnostics and therapeutics.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Enables site-specific attachment of payloads (e.g., toxins, dyes, drugs) to antibodies, proteins, and peptides via its bioorthogonal tetrazine and alkyne groups.
• Fluorescent Probe and Diagnostic Agent Synthesis: Used to construct imaging agents for in vitro and in vivo diagnostics, leveraging the rapid and specific inverse electron-demand Diels-Alder (IEDDA) reaction of the tetrazine moiety.
• Polymer and Biomaterial Functionalization: The hydrophilic PEG5 spacer improves solubility, making this reagent ideal for modifying surfaces, hydrogels, and nanoparticles for biomedical applications.
• Chemical Biology and Proteomics: Facilitates the labeling and study of biomolecules in live cells or complex biological mixtures without interfering with native biochemical processes.
• Pre-targeting Strategies in Radioimmunotherapy: A key component in multi-step pre-targeting approaches to deliver radioactive isotopes with high specificity and reduced off-target exposure.

Basic Information

Product Name	Methyltetrazine-Peg5-Alkyne
CAS No.	1802907-97-6
Molecular Formula	C27H44N6O8
Molecular Weight	580.68 g/mol
Synonyms	Methyltetrazine-PEG5-Alkyne; 3-Methyl-6-(1,2,4,5-tetrazin-3-yl)-1,2,4,5-tetrazine-PEG5-alkyne; TZ-PEG5-Alkyne; Methyl-Tetrazine-PEG5-Propargyl; Alkyne-PEG5-Methyltetrazine; 1,2,4,5-Tetrazine-3-methyl-PEG5-propargyl ether; Propargyl-PEG5-Methyl-1,2,4,5-tetrazine
EINECS	Contact for details

Quality Control

Our Methyltetrazine-Peg5-Alkyne is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive research and development applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Red to purple solid or oil
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, water, and common organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.