Description

H-Lys(Dabsyl)-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Gln-Lucifer Yellow is a specialized, custom-labeled peptide derivative designed for advanced biochemical research and diagnostic applications. This compound matters for its unique dual-labeling system, combining the fluorescent Lucifer Yellow chromophore with the Dabsyl (4-dimethylaminoazobenzene-4'-sulfonyl) quenching group, enabling sophisticated fluorescence resonance energy transfer (FRET) studies and protease activity assays. It is essential for researchers and developers in pharmaceutical discovery, proteomics, and clinical diagnostics who require high-purity, well-characterized tools for studying enzyme kinetics, protein-protein interactions, and developing novel assay platforms.

Application

• FRET-Based Protease Assays: Serves as a highly sensitive intramolecularly quenched fluorescent substrate for monitoring the activity of proteolytic enzymes like caspases, thrombin, or furin.
• Enzyme Kinetics & Mechanism Studies: Used to determine kinetic parameters (Km, kcat) and elucidate the catalytic mechanisms of specific peptidases.
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify and characterize novel inhibitors or activators of target proteases.
• Biomarker Detection & Diagnostic Development: Functions as a key component in the development of fluorescent diagnostic kits for detecting specific enzyme activities associated with diseases.
• Cell Biology & Imaging: Can be utilized in cellular assays to visualize and quantify protease activity in real-time within live or fixed cells.
• Peptide Substrate Specificity Profiling: Aids in mapping the cleavage site preferences and specificity of newly discovered or engineered proteases.

Basic Information

Product Name	H-Lys(Dabsyl)-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Gln-Lucifer Yellow
CAS No.	1802078-42-7
Molecular Formula	C₁₀₀H₁₃₈N₂₈O₃₄S₂ (Contact for exact, mass-spec confirmed formula)
Molecular Weight	~2280.5 g/mol (Theoretical average)
Synonyms	Dabsyl-Lucifer Yellow Labeled Peptide; Lys(Dabsyl)-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Gln-Lucifer Yellow; Intramolecularly Quenched Fluorescent Peptide Substrate; FRET Peptide Substrate; Caspase Substrate Analog; Lucifer Yellow CH-labeled peptide; DABCYL-Lucifer Yellow FRET Pair Conjugate; Custom Labeled Peptide 1802078-42-7
EINECS	Not listed / Contact for details

Quality Control

Every batch of our custom peptides is synthesized and purified to meet exacting standards for research use. Rigorous quality testing is performed, including reverse-phase HPLC for purity assessment and mass spectrometry (MS) for identity confirmation. Certificates of Analysis (COA) detailing purity (typically >95% by HPLC), molecular weight verification, and other relevant analytical data are provided with each shipment to ensure traceability and reliability for your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into single-use quantities to minimize freeze-thaw cycles.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.