Description

Ac-Lys-Gln-Leu-Arg-Afc CAS NO 1802078-38-1 is a synthetic peptide derivative, specifically a fluorogenic substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and developers requiring precise detection and quantification of specific protease activity. It is primarily utilized in the life sciences sector for biochemical research, drug discovery, and diagnostic assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the specific detection and kinetic analysis of target proteases in research settings.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery platforms to identify and characterize potential protease inhibitors or activators.
• Biochemical Research: Employed in mechanistic studies to understand protease function, substrate specificity, and enzymatic pathways.
• Diagnostic Development: Serves as a key component in the development of sensitive in-vitro diagnostic tests for diseases associated with dysregulated protease activity.
• Cell Biology Studies: Applied in assays to monitor protease activity within cellular environments or in cell lysates.
• Quality Control in Biologics: Potential use in monitoring the activity or stability of enzyme-based therapeutic products during manufacturing.

Basic Information

Product Name	Ac-Lys-Gln-Leu-Arg-Afc
CAS No.	1802078-38-1
Molecular Formula	C34H55N11O9
Molecular Weight	761.88 g/mol
Synonyms	Acetyl-Lysyl-Glutaminyl-Leucyl-Arginyl-7-Amino-4-Trifluoromethylcoumarin; Ac-KQLR-AFC; Ac-Lys-Gln-Leu-Arg-7-Amino-4-Trifluoromethylcoumarin; Fluorogenic Peptide Substrate Ac-KQLR-AFC; Protease Substrate 1802078-38-1; KQLR-AFC (acetylated); AFC-labeled peptide substrate
EINECS	Contact for details

Quality Control

Our Ac-Lys-Gln-Leu-Arg-Afc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry (MS) for identity confirmation. We ensure compliance with relevant standards for research-grade biochemicals. A detailed Certificate of Analysis (COA) documenting purity, identity, and performance in standard assay conditions is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Functional Activity	Conforms to reference in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.