Description

Dabcyl-(Asn670,Leu671)-Amyloid Beta/A4 Protein Precursor770 (669-674)-Edans is a specialized, high-purity fluorogenic peptide substrate designed for protease activity assays. This compound is critical for biochemical research focused on the proteolytic processing of the amyloid precursor protein (APP), a key pathway in Alzheimer's disease studies. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and diagnostic developers requiring precise, sensitive tools for enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: Specifically designed as a substrate for β-secretase (BACE1) and other proteases involved in APP cleavage.
• Alzheimer's Disease Research: Enables the study of amyloid-beta peptide generation and the molecular mechanisms underlying disease pathology.
• High-Throughput Screening (HTS): Used in drug discovery campaigns to identify and characterize potential BACE1 inhibitors for therapeutic development.
• Enzyme Kinetics Studies: Facilitates the determination of kinetic parameters (Km, Vmax, kcat) for proteases due to its FRET-based signal generation.
• Biomarker Development: Serves as a core component in developing in vitro diagnostic assays for protease activity in biological samples.
• Cell-Based Assays: Applicable in cellular models to monitor APP processing and γ-secretase activity in real-time or endpoint formats.

Basic Information

Product Name	Dabcyl-(Asn670,Leu671)-Amyloid Beta/A4 Protein Precursor770 (669-674)-Edans
CAS No.	1802078-37-0
Molecular Formula	C58H84N14O19S2
Molecular Weight	1305.5 g/mol
Synonyms	Dabcyl-SEVNLDA-Edans; FRET Substrate APPsw; BACE1 Substrate, Fluorogenic; Amyloid Precursor Protein (669-674) FRET Peptide; (7-Methoxycoumarin-4-yl)acetyl-SEVNLDA-(4-(4-dimethylaminophenyl)diazenyl)benzoyl; APP Secretase Substrate; γ-Secretase Substrate IV; β-Secretase Substrate; Dabcyl-SEVNLDA-Edans Fluorogenic Peptide
EINECS	Contact for details

Quality Control

Every batch of this fluorogenic peptide is manufactured and analyzed under strict quality protocols to ensure high purity and consistent performance in sensitive biochemical assays. Our quality system includes identity confirmation by mass spectrometry (MS) and HPLC, purity assessment by analytical HPLC, and functional validation in enzymatic assays. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data is provided with each shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%
Functional Activity	Cleavage by BACE1 generates a dose-dependent increase in fluorescence
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.