Description

Dabcyl-(Asn670,Leu671)-Amyloid Beta/A4 Protein Precursor770 (667-675)-Edans is a specialized, high-purity peptide substrate designed for advanced biochemical research and diagnostic assay development. This compound is critically important as a fluorogenic substrate for the study of proteolytic enzymes, particularly those involved in Alzheimer's disease research, such as β-secretase (BACE-1). It is an essential tool for pharmaceutical R&D teams, diagnostic kit manufacturers, and academic laboratories focused on neurodegenerative disease mechanisms, high-throughput screening, and enzyme kinetics.

Application

• β-Secretase (BACE-1) Activity Assays: Primary substrate for fluorometric and kinetic studies of BACE-1 enzyme activity, a key target in Alzheimer's disease therapeutic research.
• High-Throughput Screening (HTS): Enables the screening of large compound libraries for potential BACE-1 inhibitors in drug discovery pipelines.
• Diagnostic Kit Development: Used as a core component in the development of sensitive in vitro diagnostic (IVD) assays for measuring protease activity in biological samples.
• Enzyme Kinetics & Mechanism Studies: Facilitates detailed kinetic analysis (Km, Vmax, kcat) of amyloid precursor protein (APP) cleaving proteases.
• Biomarker Research: Supports research into proteolytic biomarkers associated with neurodegenerative conditions and other diseases.
• Cell-Based Assay Systems: Applicable in cellular models to study APP processing and the effects of pharmacological modulators in a more physiologically relevant context.

Basic Information

Product Name	Dabcyl-(Asn670,Leu671)-Amyloid Beta/A4 Protein Precursor770 (667-675)-Edans
CAS No.	1802078-36-9
Molecular Formula	C73H104N18O22S2
Molecular Weight	1593.86 g/mol
Synonyms	Dabcyl-SEVNLDAEFK-Edans; BACE-1 Substrate, Fluorogenic; Dabcyl-SEVNLDAEFK(Edans); APP770 (667-675) Fluorogenic Peptide Substrate; β-Secretase Substrate IV; (7-Methoxycoumarin-4-yl)acetyl-SEVNLDAEFK(Dabcyl); SEVNLDAEFK Dabcyl/Edans Substrate; Amyloid Precursor Protein Cleavage Site Peptide
EINECS	Contact for details

Quality Control

Every batch of Dabcyl-(Asn670,Leu671)-Amyloid Beta/A4 Protein Precursor770 (667-675)-Edans is manufactured and tested under stringent quality management systems to ensure exceptional purity, identity, and performance consistency for critical research applications. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed HPLC purity, MS confirmation, and functional activity data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, consider aliquoting to minimize freeze-thaw cycles and maintain optimal stability. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized solid
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area % at 220 nm & 280 nm)
Peptide Content	≥ 70% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Cleavage by recombinant BACE-1 generates fluorescent signal (λex 340 nm, λem 490 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.