Description

Exo-Bcn-Peg4-Nhs Ester CAS NO 1702356-19-1 is a heterobifunctional crosslinking reagent featuring an exo-BCN (bicyclo[6.1.0]nonyne) group and an amine-reactive NHS ester, connected by a tetraethylene glycol (PEG4) spacer. This compound is engineered for efficient, copper-free click chemistry bioconjugation, enabling the stable and specific labeling of biomolecules under mild, physiological conditions. It is an essential tool for researchers and developers in biopharmaceuticals, proteomics, and diagnostic assay development who require precise and reliable protein modification, antibody-drug conjugate (ADC) synthesis, and surface functionalization.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: Site-specific conjugation of cytotoxic payloads to antibodies via the exo-BCN handle for targeted cancer therapies.
• Protein and Peptide Labeling: Modification of lysine residues with fluorophores, biotin, or other probes for imaging, detection, and pull-down assays.
• Surface Functionalization: Immobilization of biomolecules onto azide-modified biosensors, microarrays, or nanoparticles to create bioactive surfaces.
• Lipid and Polymer Conjugation: Introduction of bioorthogonal handles into liposomes or polymeric drug delivery systems for subsequent targeting ligand attachment.
• Live-Cell Labeling: Tagging of cell surface proteins expressing azido groups via strain-promoted alkyne-azide cycloaddition (SPAAC) for real-time imaging.
• Diagnostic Reagent Development: Creating stable conjugates for immunoassays (ELISA, lateral flow) and molecular imaging probes.

Basic Information

Product Name	Exo-Bcn-Peg4-Nhs Ester
CAS No.	1702356-19-1
Molecular Formula	C31H46N4O10
Molecular Weight	634.72 g/mol
Synonyms	exo-BCN-PEG4-NHS ester; (1R,8S,9S)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3,6,9,12-tetraoxapentadecan-15-yl) carbamate; BCN-PEG4-NHS; exo-BCN-PEG4-NHS Ester; BCN-PEG4-NHS Ester; exo-BCN-PEG4-N-hydroxysuccinimide ester; exo-BCN-PEG4-OSu; Bicyclo[6.1.0]nonyne-PEG4-NHS Ester
EINECS	Contact for details

Quality Control

Our Exo-Bcn-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided to guarantee traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.