Description

Propargyl-Peg5-1-o-(B-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite is a high-purity phosphoramidite monomer specifically designed for the solid-phase synthesis of oligonucleotides and other biomolecules. This compound is critical for introducing a propargyl group via a stable, hydrophilic polyethylene glycol (PEG5) linker, enabling subsequent bioorthogonal click chemistry modifications such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is an essential building block for researchers and manufacturers in pharmaceutical development, diagnostic probe synthesis, and advanced materials science, where precise functionalization of nucleic acids is required.

Application

• Oligonucleotide Synthesis: Key monomer for the automated solid-phase synthesis of DNA/RNA strands bearing a terminal alkyne handle for post-synthetic conjugation.
• Probe and Diagnostic Development: Used to create labeled probes for fluorescence in situ hybridization (FISH), microarrays, and other detection platforms by attaching fluorophores, quenchers, or other reporter molecules.
• Therapeutic Oligonucleotide Manufacturing: Enables site-specific attachment of targeting ligands (e.g., peptides, antibodies) or pharmacokinetic enhancers (e.g., lipids, polymers) to siRNA, antisense oligonucleotides (ASOs), and aptamers.
• Bioconjugation and Immobilization: Facilitates the covalent attachment of oligonucleotides to surfaces, nanoparticles, polymers, or proteins for biosensor and microarray fabrication.
• Materials Science: Serves as a building block for creating DNA-functionalized hydrogels, nanomaterials, and other smart materials with programmable properties.
• Chemical Biology Research: A vital tool for studying nucleic acid-protein interactions, developing new bioassays, and engineering synthetic biological circuits.

Basic Information

Product Name	Propargyl-Peg5-1-o-(B-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite
CAS No.	1682657-14-2
Molecular Formula	C31H52N3O8P
Molecular Weight	625.74 g/mol
Synonyms	Propargyl-PEG5-phosphoramidite; 5'-Propargyl Hexaethylene Glycol Phosphoramidite; Propargyl-hexa(ethylene glycol) phosphoramidite; 2-Cyanoethyl N,N-diisopropylchlorophosphoramidite derivative of propargyl-PEG5-alcohol; Propargyl-PEG5-CPG precursor; Alkyne-PEG5-phosphoramidite; 1-O-(2-Cyanoethoxy-(diisopropylamino)phosphanyl)-2,5,8,11,14-pentaoxaheptadec-16-yn-17-ol
EINECS	Contact for details

Quality Control

Every batch of our Propargyl-Peg5-1-o-(B-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite is manufactured under strict quality management systems and undergoes rigorous analytical testing to ensure it meets the high standards required for sensitive oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and moisture content. Our quality protocols are designed to support GMP-grade applications in therapeutic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.