Description

UV Cleavable Biotin-PEG2-Azide is a specialized heterobifunctional crosslinker designed for controlled bioconjugation and affinity purification. This compound integrates a biotin moiety for strong streptavidin binding, a UV-cleavable linker for on-demand elution, and an azide group for bioorthogonal click chemistry via copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is essential for researchers and manufacturers in proteomics, drug discovery, and diagnostic assay development who require precise, reversible capture and release of biomolecules.

Application

• Affinity Purification & Pull-Down Assays: Immobilization of biotinylated targets onto streptavidin resins, followed by gentle UV-light-mediated elution to preserve protein integrity.
• Proteomic Profiling: Labeling and isolation of specific protein classes or post-translational modifications for mass spectrometry analysis.
• Chemical Biology & Target Identification: Synthesis of activity-based probes (ABPs) for labeling and identifying enzyme active sites in complex biological systems.
• Diagnostic Kit Development: Construction of sensitive detection platforms, such as ELISA or lateral flow assays, utilizing the biotin-streptavidin signal amplification system.
• ADC (Antibody-Drug Conjugate) & Bioconjugation Research: Site-specific conjugation of payloads, dyes, or other functional molecules to antibodies or proteins.
• Surface Functionalization: Modification of nanoparticles, chips, or beads with biotin for subsequent capture and UV-triggered release studies.

Basic Information

Product Name	UV Cleavable Biotin-PEG2-Azide
CAS No.	1654739-36-2
Molecular Formula	C24H41N7O7S
Molecular Weight	571.70 g/mol
Synonyms	Biotin-PEG2-Azide, UV Cleavable; 2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethyl 5-azidopentanoate; Biotin-PEG2-Azide (UV Cleavable); Cleavable Biotin-Azide Reagent; UV-Sensitive Biotin-Azide Crosslinker; Biotinylated Azide Photocleavable Linker; Photo-Releasable Biotin-N3
EINECS	Contact for details

Quality Control

Our UV Cleavable Biotin-PEG2-Azide is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and functional performance are provided to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Functional Performance	UV Cleavage Efficiency ≥90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.