Description

Tco-Peg4-Nhs Ester CAS NO 1621096-79-4 is a heterobifunctional crosslinking reagent featuring a thermolabile TCO (trans-cyclooctene) group and an amine-reactive NHS (N-hydroxysuccinimide) ester, connected by a tetraethylene glycol (PEG4) spacer. This compound is engineered for efficient, rapid, and bioorthogonal conjugation in aqueous environments, enabling the stable labeling of biomolecules. It is an essential tool for researchers and developers in bioconjugation, antibody-drug conjugate (ADC) development, proteomics, and in vivo imaging, where precise and fast click chemistry is required.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via bioorthogonal TCO-tetrazine click chemistry.
• Protein and Peptide Labeling: Enables the modification of lysine residues with TCO handles for subsequent attachment of fluorescent dyes, affinity tags, or other functional molecules.
• Surface Functionalization: Used to introduce TCO groups onto nanoparticles, biosensors, or microarray slides for the capture and detection of tetrazine-modified analytes.
• In Vivo Pretargeting Strategies: A key component in multi-step pretargeting approaches for radioimmunotherapy and diagnostic imaging, improving target-to-background ratios.
• Polymer and Biomaterial Modification: Facilitates the crosslinking or functionalization of hydrogels and polymeric materials with bioactive ligands.
• Chemical Biology Probes: Serves as a critical reagent for creating activity-based probes to study enzyme function or protein-protein interactions.

Basic Information

Item	Details
Product Name	Tco-Peg4-Nhs Ester
CAS No.	1621096-79-4
Molecular Formula	C₂₉H₄₀N₂O₁₀
Molecular Weight	576.64 g/mol
Synonyms	trans-Cyclooctene-PEG4-NHS Ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3,6,9,12-tetraoxapentadecan-15-yl) carbonate; TCO-PEG4-NHS; TCO-PEG4-NHS ester; TCO-PEG4-SCM; TCO-PEG4-Osu; TCO-PEG4-OSu; TCO-PEG4-N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Every batch of Tco-Peg4-Nhs Ester is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure high purity and batch-to-batch consistency. Our quality protocols are designed to meet the stringent requirements of research and pre-clinical development. A detailed Certificate of Analysis (COA) providing purity, identity, and stability data is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.