Description

Propargyl-Peg4-Maleimide is a heterobifunctional crosslinker featuring a maleimide group for thiol conjugation and a propargyl group for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. This dual functionality makes it an essential tool for the site-specific, sequential bioconjugation of biomolecules such as proteins, peptides, and oligonucleotides. It is primarily used by researchers and process developers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay formulation.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the controlled, multi-step assembly of antibody-toxin conjugates.
• Protein and Peptide Labeling: Enables site-specific attachment of fluorophores, biotin, or other probes via orthogonal click chemistry.
• Surface Functionalization: Used to modify nanoparticles, biosensors, or microarray slides with bioactive ligands.
• Polymer and Dendrimer Synthesis: Serves as a building block for creating functionalized, biocompatible PEG-based architectures.
• Diagnostic Reagent Development: For constructing stable immunoconjugates used in ELISA, lateral flow assays, and other diagnostic platforms.
• Chemical Biology Tool: Facilitates the study of protein-protein interactions and cellular trafficking through bioorthogonal labeling strategies.

Basic Information

Product Name	Propargyl-Peg4-Maleimide
CAS No.	1609651-90-2
Molecular Formula	C20H31NO9
Molecular Weight	429.46 g/mol
Synonyms	Propargyl-PEG4-maleimide; Maleimide-PEG4-propargyl; Propargyl-PEG4-MAL; 3-(2-{2-[2-(2-{3-[(Prop-2-yn-1-yloxy)carbonyl]-2,5-dihydro-1H-pyrrol-1-yl}ethoxy)ethoxy]ethoxy}ethoxy)propanoic Acid; 1-[2-[2-[2-[2-(2-Propyn-1-yloxy)ethoxy]ethoxy]ethoxy]ethyl]-1H-pyrrole-2,5-dione; Propargyl-PEG4-MAL Crosslinker; Alkyne-PEG4-Maleimide; Click Chemistry PEG Crosslinker
EINECS	Contact for details

Quality Control

Our Propargyl-Peg4-Maleimide is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for research and bioconjugation applications. Each lot is characterized by NMR, HPLC, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous oil or solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.