Description

Endo-Bcn-Peg8-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an endo-BCN group and an NHS ester, designed for efficient bioconjugation under mild conditions. This compound is critical for creating stable, site-specific linkages between biomolecules and surfaces, nanoparticles, or other functional molecules in advanced research and development. It is primarily needed by scientists and manufacturers in the fields of biopharmaceuticals, proteomics, diagnostics, and materials science for applications such as ADC development, hydrogel formation, and surface functionalization.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry.
• PEGylation and Bioconjugation: To introduce hydrophilic PEG spacers onto peptides, proteins, or small molecules, improving solubility, stability, and pharmacokinetic profiles.
• Surface Functionalization: For modifying nanoparticles, biosensors, or microarray slides with bioactive ligands to create specific binding platforms.
• Hydrogel and Biomaterial Synthesis: As a crosslinking agent to create tunable, biocompatible networks for 3D cell culture, drug delivery, and tissue engineering.
• Diagnostic Reagent Preparation: In the development of fluorescent probes, imaging agents, and immunoassay components requiring stable, clickable linkages.
• Chemical Biology and Proteomics: For labeling, pull-down assays, and mapping protein-protein interactions using bioorthogonal chemistry.

Basic Information

Product Name	Endo-Bcn-Peg8-Nhs Ester
CAS No.	1608140-48-2
Molecular Formula	C45H73N3O19
Molecular Weight	960.1 g/mol
Synonyms	endo-BCN-PEG8-NHS Ester; endo-BCN-PEG8-NHS; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3,6,9,12,15,18,21,24-octaoxahexacosan-26-yl) carbamate; 2,5-Dioxopyrrolidin-1-yl 3,6,9,12,15,18,21,24-octaoxa-27-oxo-28-(((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)methoxy)-28-oxooctacosan-1-oate; BCN-PEG8-NHS Ester; endo-BCN-PEG8-OSu; BCN-PEG8-NHS
EINECS	Contact for details

Quality Control

Our Endo-Bcn-Peg8-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis of the NHS ester moiety.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay	≥ 90% (by NHS ester hydrolysis)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.