Description

Iodoacetamide-Peg3-Azide is a heterobifunctional crosslinking reagent designed for advanced bioconjugation and chemical biology applications. Its core value lies in combining a highly reactive iodoacetamide group for thiol-specific alkylation with a hydrophilic PEG3 spacer and a terminal azide for copper-free click chemistry. This trifunctional architecture makes it an essential tool for researchers and manufacturers in the pharmaceutical and biotechnology sectors who require precise, stable, and site-specific modification of proteins, peptides, and other biomolecules.

Application

• Site-Specific Protein Labeling: Covalent attachment of fluorophores, biotin, or other probes to cysteine residues via the iodoacetamide group, followed by click chemistry conjugation.
• Antibody-Drug Conjugate (ADC) Development: Facilitating the construction of novel ADC linkers by conjugating cytotoxic payloads to monoclonal antibodies.
• Surface Functionalization: Modifying nanoparticles, biosensors, or microarray surfaces with biomolecules for enhanced targeting and detection.
• Polymer and Material Science: Serving as a key monomer or crosslinker for synthesizing functionalized hydrogels and smart polymers.
• Chemical Proteomics: As an activity-based probe for profiling enzyme families or studying protein-protein interactions.
• Diagnostic Reagent Synthesis: Creating stable conjugates for immunoassays, lateral flow tests, and other diagnostic platforms.

Basic Information

Product Name	Iodoacetamide-Peg3-Azide
CAS No.	1594986-04-5
Molecular Formula	C11H20IN5O4
Molecular Weight	405.21 g/mol
Synonyms	2-(2-(2-(2-Azidoethoxy)ethoxy)ethoxy)-N-(2-iodoacetyl)acetamide; Iodoacetamide-PEG3-Azide; Azido-PEG3-iodoacetamide; N-(2-Iodoacetyl)-2,2'-(ethylenedioxy)bis(ethylamine) N-azide; IAA-PEG3-N3; PEG3 Iodoacetamide Azide; 1-Azido-3,6,9-trioxa-12-aza-14-oxopentadecan-15-yl iodide
EINECS	Contact for details

Quality Control

Every batch of Iodoacetamide-Peg3-Azide is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, NMR, and Mass Spectrometry analysis. Our quality commitment ensures the reagent meets the stringent requirements for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.