Description

(2E)-Tco-Pnb Ester CAS NO 1580501-97-8 is a specialized chemical building block featuring a trans-cyclooctene (TCO) moiety protected with a para-nitrobenzyl (PNB) ester group. This compound is engineered for high reactivity and selectivity in advanced bioconjugation and materials science applications, particularly in bioorthogonal chemistry. It is an essential reagent for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require precise and efficient methods for labeling biomolecules, developing antibody-drug conjugates (ADCs), or creating functionalized polymers.

Application

• Bioorthogonal Click Chemistry: Serves as a key reagent for strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with tetrazines for rapid, catalyst-free labeling of biomolecules.
• Antibody-Drug Conjugate (ADC) Development: Used for the site-specific conjugation of cytotoxic payloads to monoclonal antibodies, enabling the creation of next-generation targeted cancer therapies.
• Proteomics and Biomarker Discovery: Facilitates the labeling and enrichment of proteins and peptides for mass spectrometry analysis and cellular imaging studies.
• Diagnostic Probe Synthesis: Employed in the construction of molecular imaging probes for positron emission tomography (PET) and fluorescence imaging.
• Polymer and Materials Functionalization: Enables the introduction of bio-reactive handles onto surfaces, nanoparticles, and hydrogels for creating smart biomaterials.
• Pre-clinical Research: Provides a reliable tool for academic and industrial R&D teams exploring novel drug delivery systems and chemical biology tools.

Basic Information

Product Name	(2E)-Tco-Pnb Ester
CAS No.	1580501-97-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	(2E)-trans-Cyclooctene-PNB Ester; TCO-PNB Ester; para-Nitrobenzyl (2E)-trans-Cyclooct-2-en-1-yl Carbonate; TCO-PNB Carbonate; (2E)-TCO-PNB; (2E)-trans-Cyclooct-2-en-1-yl (4-nitrobenzyl) carbonate; Bioorthogonal TCO Reagent; SPAAC Reagent
EINECS	Contact for details

Quality Control

Our (2E)-Tco-Pnb Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioorthogonal applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C to -10°C under an inert atmosphere for long-term stability. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Related Substances (HPLC)	Individual impurity ≤ 2.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.