Description

Dbco-Peg4-Acid CAS NO 1537170-85-6 is a heterobifunctional crosslinker featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and a carboxylic acid terminus for further conjugation via amide bond formation. This compound is essential for creating stable, bioorthogonal linkages in complex biomolecular assemblies without the need for cytotoxic copper catalysts. It is a critical reagent for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced materials science.

Application

• Bioconjugation and ADC Linker Chemistry: Site-specific conjugation of payloads (e.g., drugs, toxins, fluorophores) to antibodies, proteins, or peptides via the DBCO-azide click reaction and subsequent acid-mediated coupling.
• Polymer and Biomaterial Functionalization: Modification of polymer backbones or hydrogel matrices to introduce bioorthogonal handles for subsequent immobilization of biomolecules.
• Surface Engineering and Biosensor Development: Functionalization of chips, nanoparticles, or other solid supports for creating diagnostic or research platforms.
• Chemical Biology and Proteomics: Labeling and pull-down of azide-tagged biomolecules in live cells or complex biological mixtures for tracking and analysis.
• Nanoparticle Targeting: Construction of targeted drug delivery systems by conjugating targeting ligands to nanoparticle surfaces.
• Oligonucleotide Modification: Synthesis of modified nucleic acids (DNA/RNA) for therapeutic or diagnostic applications.

Basic Information

Product Name	Dbco-Peg4-Acid
CAS No.	1537170-85-6
Molecular Formula	C33H41N3O8
Molecular Weight	607.70 g/mol
Synonyms	DBCO-PEG4-COOH; DBCO-PEG4-Acid; DBCO-PEG4-Acid; Dibenzocyclooctyne-PEG4-Acid; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (2,5,8,11-tetraoxatetradecan-14-yl) carbamate; DBCO-PEG4-Carboxylic Acid; DBCO-PEG4-COOH; Azadibenzocyclooctyne-PEG4-Acid
EINECS	Contact for details

Quality Control

Our Dbco-Peg4-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.