Description

Azido-PEG1-CH2CO2-NHS is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a click chemistry-ready azide group, connected by a short polyethylene glycol (PEG) spacer. This compound is engineered to facilitate efficient and selective bioconjugation, enabling the stable attachment of biomolecules, polymers, or surfaces under mild, aqueous conditions. It is an essential tool for researchers and manufacturers in biopharmaceutical development, proteomics, diagnostics, and materials science who require precise and reliable crosslinking strategies.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via click chemistry.
• PEGylation of Peptides and Proteins: To introduce a short PEG linker for improving solubility, stability, and pharmacokinetic properties.
• Surface Functionalization: For modifying nanoparticles, biosensors, or microarray slides with azide groups for subsequent bio-orthogonal labeling.
• Polymer Chemistry: As a building block for synthesizing functionalized polymers and hydrogels with clickable handles.
• Fluorescent Probe and Biotin Labeling: To attach dyes, biotin, or other reporter molecules to primary amines on target biomolecules.
• Protein-Protein Crosslinking: For studying protein interactions or creating stable protein complexes.

Basic Information

Product Name	Azido-PEG1-CH2CO2-NHS
CAS No.	1480545-09-2
Molecular Formula	C9H12N4O5
Molecular Weight	256.22 g/mol
Synonyms	Azido-PEG1-acetic acid NHS ester; 2-[2-(2-Azidoethoxy)ethoxy]acetic acid N-hydroxysuccinimide ester; NHS-PEG1-Azide; N-(Azido-PEG1)-NHS ester; Azido-PEG1-NHS ester; 2,5-Dioxopyrrolidin-1-yl 2-[2-(2-azidoethoxy)ethoxy]acetate; Azido-OEG1-NHS ester; Azido-EG1-NHS ester
EINECS	Contact for details

Quality Control

Every batch of Azido-PEG1-CH2CO2-NHS is manufactured under strict quality management protocols and undergoes comprehensive analytical testing to ensure high purity and consistent performance. Our standard quality commitment includes verification of identity, purity, and functional group integrity. Certificates of Analysis (COA) detailing specifications and test results (including HPLC, NMR, and FTIR) are provided with each shipment and are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term stability, store in a freezer and allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.