Description

Dbco-Peg4-Maleimide CAS NO 1480516-75-3 is a heterobifunctional crosslinker featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and a maleimide group for thiol conjugation. This compound is essential for creating stable, site-specific bioconjugates without the need for cytotoxic copper catalysts, enabling advanced research and development. It is primarily needed by scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and the synthesis of functionalized nanoparticles and biomaterials.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via thiol-maleimide chemistry.
• PEGylation Reagent: For introducing polyethylene glycol (PEG) chains onto proteins, peptides, or surfaces to improve solubility, stability, and pharmacokinetics.
• Biomaterial Functionalization: For modifying hydrogels, nanoparticles, or diagnostic surfaces with bioactive ligands for targeted delivery or sensing.
• Protein/Peptide Labeling: For attaching fluorescent dyes, affinity tags (biotin), or other probes to biomolecules in a controlled, two-step manner.
• Click Chemistry Tool: As a key reagent in copper-free click chemistry workflows for labeling azide-modified biomolecules in live cells or sensitive systems.
• Diagnostic Assay Development: For constructing immunoassays or biosensors by linking detection components with high specificity and efficiency.

Basic Information

Product Name	Dbco-Peg4-Maleimide
CAS No.	1480516-75-3
Molecular Formula	C33H44N2O10
Molecular Weight	628.71 g/mol
Synonyms	DBCO-PEG4-MAL; DBCO-PEG4-Maleimide; Dibenzocyclooctyne-PEG4-Maleimide; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-(2-{2-[2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy]ethoxy}ethyl)carbamate; 1-(17-{2-[2-(2-{2-[(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethoxy]-2-oxoethoxy}ethoxy)ethoxy]ethoxy}-17-oxo-3,6,9,12-tetraoxa-16-azaheptadecyl)-1H-pyrrole-2,5-dione; DBCO-PEG4-MAL Crosslinker; Azadibenzocyclooctyne-PEG4-Maleimide
EINECS	Contact for details

Quality Control

Our Dbco-Peg4-Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.