Description

Azadibenzocyclooctyne-Conh-n-Hydroxysuccinimidyl Ester is a highly reactive, amine-reactive bioconjugation reagent designed for strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. This compound is critical for enabling fast, catalyst-free, and bioorthogonal labeling of biomolecules such as proteins, antibodies, and oligonucleotides under mild physiological conditions. It is an essential tool for researchers and developers in pharmaceutical R&D, proteomics, diagnostic assay development, and advanced materials science who require efficient and specific conjugation strategies.

Application

• Bioconjugation of Biomolecules: Primary application for labeling proteins, peptides, antibodies, and nucleic acids with azide-tagged probes, dyes, or solid supports.
• Pharmaceutical Research: Synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutic constructs via site-specific modification.
• Diagnostic Development: Conjugation of detection molecules (e.g., enzymes, fluorophores) for use in immunoassays, flow cytometry, and in vitro diagnostics (IVD).
• Proteomics & Cell Biology: Labeling of cell surface glycans or metabolically incorporated azido-sugars for imaging and pull-down assays.
• Materials Science: Functionalization of polymers, nanoparticles, and surfaces with bioactive ligands for creating smart biomaterials.
• Chemical Biology Tool Development: A key reagent for creating novel chemical probes to study biological processes in live cells.

Basic Information

Product Name	Azadibenzocyclooctyne-Conh-n-Hydroxysuccinimidyl Ester
CAS No.	1435934-53-4
Molecular Formula	C₂₀H₁₃N₃O₄
Molecular Weight	359.34 g/mol
Synonyms	ADIBO-NHS Ester; ADIBO-SE; Azadibenzocyclooctyne NHS Ester; 3-(3-(6,7-Dihydro-5H-dibenzo[a,c][7]annulen-6-yl)-1H-1,2,3-triazol-4-yl)propanoic Acid N-Hydroxysuccinimide Ester; 6-(4-(3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)-1H-1,2,3-triazol-1-yl)-6,7-dihydro-5H-dibenzo[a,c][7]annulene; SPAAC Reagent NHS Ester; DBCO Analogue NHS Ester
EINECS	Contact for details

Quality Control

Our Azadibenzocyclooctyne-NHS Ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We adhere to industry-standard analytical methods to guarantee product performance in your bioconjugation workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment. For long-term stability, store under inert atmosphere (argon or nitrogen) and allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.