Description

Propargyl-Peg4-5-Nitrophenyl Carbonate is a heterobifunctional crosslinker and labeling reagent featuring a propargyl group for copper-catalyzed azide-alkyne cycloaddition (CuAAC) and a reactive 5-nitrophenyl carbonate ester for amine modification. This compound is essential for creating stable, site-specific bioconjugates, enabling the precise attachment of biomolecules, polymers, or probes. It is primarily needed by researchers and manufacturers in pharmaceutical development, proteomics, and advanced materials science for constructing antibody-drug conjugates (ADCs), PEGylated therapeutics, and functionalized surfaces.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of cytotoxic drugs or fluorescent dyes to antibodies, proteins, or peptides via amine coupling and click chemistry.
• PEGylation Reagent: Functionalization of poly(ethylene glycol) (PEG) chains with clickable handles for subsequent attachment to therapeutic molecules or nanoparticles.
• Surface Functionalization: Modification of amine-coated surfaces, beads, or chips with alkyne groups to create platforms for immobilizing azide-bearing biomolecules.
• Polymer Chemistry: Synthesis of functionalized block copolymers and dendrimers with orthogonal reactive groups for advanced drug delivery systems.
• Probe and Sensor Synthesis: Construction of molecular probes for activity-based protein profiling (ABPP) and diagnostic assays.
• Chemical Biology Tool: A key reagent for studying protein-protein interactions, cellular trafficking, and biomolecular dynamics through bioorthogonal labeling.

Basic Information

Product Name	Propargyl-Peg4-5-Nitrophenyl Carbonate
CAS No.	1422540-81-5
Molecular Formula	C₂₂H₂₉NO₁₁
Molecular Weight	507.47 g/mol
Synonyms	Propargyl-PEG4-5-nitrophenyl carbonate; 5-Nitrophenyl Carbonate-PEG4-Propargyl; Propargyl-PEG4-NPC; 2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2-(2-(prop-2-yn-1-yloxy)ethoxy)ethoxy)ethoxy)ethoxy)propanoate (common misassignment check); 4-Nitrophenyl PEG4 propargyl carbonate; Propargyloxy-PEG4-5-nitrophenyl carbonate; Alkyne-PEG4-NPC; NPC-PEG4-Propargyl
EINECS	Contact for details

Quality Control

Our Propargyl-Peg4-5-Nitrophenyl Carbonate is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.