Description

5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)-n-(2-(2-(2-(Prop-2-Yn-1-Yloxy)Ethoxy)Ethoxy)Ethyl)Pentanamide is a sophisticated biotin derivative featuring a polyethylene glycol (PEG) linker terminated with a reactive alkyne group. This compound is engineered for high-value applications in bioconjugation and chemical biology, enabling the precise labeling and functionalization of biomolecules. It is primarily utilized by researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors for developing targeted therapeutics and advanced analytical probes.

Application

• Bioconjugation Reagent: Used for site-specific labeling of proteins, antibodies, and peptides via click chemistry (CuAAC) reactions.
• Proteomics Research: Acts as a critical tool for affinity purification, pull-down assays, and studying protein-protein interactions through its high-affinity binding to streptavidin/avidin.
• Diagnostic Assay Development: Incorporated into the design of ELISA, lateral flow assays, and other diagnostic platforms for signal amplification and detection.
• Drug Delivery Systems: Serves as a linker in the synthesis of antibody-drug conjugates (ADCs) and other targeted drug delivery vehicles.
• Surface Functionalization: Used to modify nanoparticles, biosensors, and microarray surfaces with biotin for subsequent capture or detection steps.
• Chemical Biology Probes: Enables the creation of activity-based probes for enzyme profiling and cellular imaging studies.

Basic Information

Product Name	5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)-n-(2-(2-(2-(Prop-2-Yn-1-Yloxy)Ethoxy)Ethoxy)Ethyl)Pentanamide
CAS No.	1421701-68-9
Molecular Formula	C21H34N4O6S
Molecular Weight	470.58 g/mol
Synonyms	Biotin-PEG3-Alkyne; Biotin-PEG3-Propargyl; Biotin-PEG3-Propargyl; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-(prop-2-yn-1-yloxy)ethoxy]ethoxy]ethyl]pentanamide; Biotin-PEG3-Alkyne Linker; Biotinylated PEG Alkyne; Alkyne-PEG3-Biotin; Biotin-PEG3-C≡CH
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical follows stringent quality protocols. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural identity and purity. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, assay results, and impurity profiles. Our quality management system is designed to support research and GMP-grade manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.