Description

6-Chloro-4-Methylumbelliferyl β-D-Glucopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of β-glucosidase enzyme activity. This compound matters for researchers and quality control labs requiring precise, high-throughput enzymatic assays due to its cleavable glycosidic bond, which releases a highly fluorescent product upon enzymatic hydrolysis. It is primarily needed by the biotechnology, pharmaceutical, and diagnostic industries for applications in enzyme kinetics, high-throughput screening (HTS), and biochemical research.

Application

• Biochemical Research & Enzyme Kinetics: As a specific substrate for studying the activity, inhibition, and kinetics of β-glucosidase enzymes.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify potential inhibitors or activators of β-glucosidase, a target for various metabolic disorders.
• Diagnostic Assay Development: Employed in the development of fluorometric diagnostic kits for detecting enzyme deficiencies, such as Gaucher disease.
• Microbiology & Cell Biology: Useful as a reporter substrate in studies involving genetically engineered cells or microorganisms expressing β-glucosidase.
• Quality Control in Biomanufacturing: For monitoring and validating the activity of enzyme preparations used in industrial biocatalysis or therapeutic protein production.
• Plant & Agricultural Research: Applied in studies of plant biochemistry to measure β-glucosidase activity involved in defense mechanisms and metabolic pathways.

Basic Information

Product Name	6-Chloro-4-Methylumbelliferyl β-D-Glucopyranoside
CAS No.	1421228-15-0
Molecular Formula	C₁₆H₁₇ClO₈
Molecular Weight	372.75 g/mol
Synonyms	6-Chloro-4-methylumbelliferyl β-D-glucoside; 6-Chloro-4-MU-β-D-glucopyranoside; 4-Methylumbelliferyl-6-chloro-β-D-glucopyranoside; β-Glucosidase substrate 6-chloro-4-methylumbelliferyl; CMU-β-Glc; 6-Cl-4-MU-β-Glc; (2R,3S,4S,5R,6S)-2-(6-Chloro-4-methyl-2-oxo-2H-chromen-7-yl)oxy-6-(hydroxymethyl)oxane-3,4,5-triol
EINECS	Contact for details

Quality Control

Our 6-Chloro-4-Methylumbelliferyl β-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications and test results are provided and available upon request to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.