Description

2,5-Dioxopyrrolidin-1-Yl 1-(1,3-Dioxoisoindolin-2-Yloxy)-3,6,9,12-Tetraoxapentadecan-15-Oate is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation and polymer chemistry. This compound matters for its ability to facilitate the precise attachment of biomolecules or functional polymers via its distinct amine-reactive and hydroxyl-reactive ester groups. It is primarily needed by researchers and manufacturers in the fields of biopharmaceutical development, proteomics, and advanced material science for creating stable, well-defined conjugates.

Application

• Bioconjugation and Protein Labeling: Used to crosslink peptides, proteins, or antibodies with other biomolecules, fluorophores, or solid supports for diagnostic assays and therapeutic applications.
• Polymer Functionalization: Serves as a key linker for modifying polyethylene glycol (PEG) derivatives or other polymers to introduce reactive handles for further chemical modification.
• Drug Delivery System Development: Enables the covalent attachment of drug payloads to targeting ligands or biodegradable polymer carriers.
• Surface Immobilization Chemistry: Facilitates the stable grafting of biomolecules onto sensor chips, nanoparticles, or chromatography resins.
• Proteomics Research: Employed in crosslinking mass spectrometry (XL-MS) studies to investigate protein-protein interactions and complex structures.
• Synthesis of Advanced Materials: Acts as a building block for creating functional hydrogels, dendrimers, or other smart materials with tailored properties.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 1-(1,3-Dioxoisoindolin-2-Yloxy)-3,6,9,12-Tetraoxapentadecan-15-Oate
CAS No.	1415328-95-8
Molecular Formula	C25H32N2O12
Molecular Weight	552.53 g/mol
Synonyms	NHS-PEG4-Phthalimide Ester; N-Hydroxysuccinimide-PEG4-Phthalimide Ester; 1-(1,3-Dioxoisoindolin-2-yloxy)-3,6,9,12-tetraoxapentadecan-15-oic acid 2,5-dioxopyrrolidin-1-yl ester; Phthalimide-PEG4-NHS Ester; 2,5-Dioxopyrrolidin-1-yl 1-(1,3-dioxoisoindolin-2-yloxy)-3,6,9,12-tetraoxapentadecan-15-oate; Heterobifunctional PEG Crosslinker, NHS/Phthalimide; PhthN-PEG4-NHS
EINECS	Contact for details

Quality Control

Our 2,5-Dioxopyrrolidin-1-Yl 1-(1,3-Dioxoisoindolin-2-Yloxy)-3,6,9,12-Tetraoxapentadecan-15-Oate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to warm to room temperature before opening to minimize moisture condensation. For long-term storage, keep containers in a dry, inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.