Description

Dbco-(Ch2)2-Nh2-Co-(Ch2)3Cooh-Nhs CAS NO 1393350-27-0 is a heterobifunctional crosslinking reagent featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound is essential for creating stable, covalent linkages between biomolecules without the need for cytotoxic copper catalysts, enabling efficient bioconjugation under physiological conditions. It is a critical tool for researchers and developers in biopharmaceuticals, diagnostic assay development, and advanced materials science, facilitating the precise labeling and functionalization of proteins, antibodies, and nanoparticles.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via amine groups.
• Protein and Peptide Labeling: Enables the attachment of fluorophores, biotin, or other probes for imaging and detection studies.
• Surface Functionalization: Used to modify nanoparticles, microspheres, or biosensor surfaces with bioactive ligands.
• Polymer and Biomaterial Modification: Incorporates functional handles into hydrogels or polymeric scaffolds for cell culture and tissue engineering.
• Diagnostic Reagent Development: Key component in creating sensitive and specific immunoassay components.
• Click Chemistry Toolkits: A fundamental reagent in bioorthogonal chemistry kits for cellular labeling and in vivo tracking.

Basic Information

Product Name	Dbco-(Ch2)2-Nh2-Co-(Ch2)3Cooh-Nhs
CAS No.	1393350-27-0
Molecular Formula	C28H29N3O6
Molecular Weight	503.55 g/mol
Synonyms	DBCO-NHS Ester; DBCO-SE; 6-((6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)amino)-6-oxohexanoic acid DBCO derivative; Dibenzocyclooctyne-N-hydroxysuccinimide ester; DBCO-PEG4-NHS ester; Azadibenzocyclooctyne NHS ester; ADIBO-NHS; DBCO-acid-NHS
EINECS	Contact for details

Quality Control

Every batch of our Dbco-(Ch2)2-Nh2-Co-(Ch2)3Cooh-Nhs is manufactured and tested under strict quality management systems. We ensure high purity and batch-to-batch consistency through comprehensive analytical techniques including HPLC, NMR, and MS. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to support your research and development compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.